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ABSTRACT

Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine. After enzymatic hydrolysis,Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Pressure PU Filter Gasket Sandal Making Machine;Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flour Making Machine Wheat Maize Flour Mill Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, 1996;Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh,1991).Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Metal Material and Sports Theme Custom Metal Medal,Portable Installation and Ce Certification 20g/Hr OEM Air Purifier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)(Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, 1996). The soluble protein may have commercial use as gelatines,Full Computer Plain Socks Knitting Machine with High Quality, peptides or amino acids production (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, 1991;New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles.Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall).Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Roller Shutter Window Torsion Springs⁰C for 24 h. The sample was then treated with 1%100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale⁰C for 2 h. After this treatment,Carbon Graphite Crucible. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Decorative Aluminum Solid Panel for Commercial Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, as shown inChicken Cage Mesh Hexagonal Wire Mesh Machine.

Di DIN3352 Double Orifice Automatic Air Release Valve Dn65⁰C and left for 2 h (Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Magic Hair Twist   Curl Sponge Brush. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Proteins were also determined by Biuret and Kjeldhal'Geyi Disposable Laparoscopic Visible Optical Trocar(Roller Wedge Screen Cylinder). The filtrate in branch B was treated in the same way as branch B inHigh Quality Air Conditioner Cover Plastic Mold. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bimetallic Thermometer Industrial Temperature Gauge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19 Network Cabinet with Lockable Rear Door(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.175mm Diamond Cutting Blade for Granite&Tiles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Freshwater Pearl Butterfly Flower Bride HairpinandEuro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. Enzymatic (Uprigth Touch-Screen Red Wine Chiller)Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator(sulfuric acid)Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder(Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, flow rate of 0.1 mL/min., and l = 620 nm.

Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard.Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Womenshows the peptides separations after enzymatic hydrolysis.Groove Type Wood Log Tree Peeling and Debarking Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElbow Forming Machine Gorelocker HVAC Duct Making Machine, but not shown inProfessional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver,High Quality CE Digital Display Adjustable Power Supply, and also free amino acids (Whitaker et al., 1963).Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens,1996).

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Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, V_e, and K_avcalculated as discussed previously.Custom Striped Compression Soccer Socks for Adults,Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bathroom False Plastic Board Interior Decorative 3D Wall Panels) (Reis et al.,1989;Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil.,1991;Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping.,1991).Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen(1992), were lower, except for cystine and tyrosine,High Tensile Strength Galvanized Steel Wire.

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CONCLUSIONS

24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price) for the financial support.

REFERENCES

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ASTM A335 Alloy Seamless Steel Pipe for Power Plant]

Brown, E. M; Thompson, C .J.,Best Price PVC Coated Fiberglass Mesh Insect Window Screen, M.M.(1994),Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application.The Journal of the American Leather Chemists Association (JALCA)89(7)[Designed Steel Frame Factory Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. Analytical Biochemistry212,436-445[By6-8-200-L Automatic Doypack Premade Pouch Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Factory OEM CNC Machined Gear Motor Shaft. Biotechnology letters 14,6, 531-534[6 Inch Plastic Breathing Cap for The IBC Valve]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Slide for Indoor Play Structure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Cookware]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tct Circular Saw Blade for Grass Cutting]

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard]

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)]

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), Part I.Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).Cute Pink Cashmere Soft Plush Lining Warm Bootie, 175-184[Direct Sales Fully Automatic 4 Heads Liquid Bottle Detergent Filling Sealing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Gold Marathon Running Sport Medal for Promotion Gift]

Whitaker,J.R.(1963),3kw 24V 310mm Vertical Agv Drive Wheel Assembly. Analytical Chemistry35(12), 1950-1953[FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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