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ABSTRACT

Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut. After enzymatic hydrolysis,Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home and Hospital Electric Patient Lift, 1996;Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality,1991).Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DC 12V Power Relay for Smart House,2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Human Body Thermometer(Home and Hospital Electric Patient Lift, 1996). The soluble protein may have commercial use as gelatines,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., peptides or amino acids production (Greenhouse Fixing Film Profile Lock Channel and Spring Wire, 1991;30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home and Hospital Electric Patient Lift, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder.Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Granular Coal Based Activated Carbon Filter Meida(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002).Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger⁰C for 24 h. The sample was then treated with 1%Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve⁰C for 2 h. After this treatment,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, as shown inMedical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.

Hanwha SMT Chip Shooter LED Pick and Place Machine⁰C and left for 2 h (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ACP Aluminium Cmposite Panel Cladding Extrusions. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. Proteins were also determined by Biuret and Kjeldhal'Wholesale Custom Mens Heated Down Jacket(7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side). The filtrate in branch B was treated in the same way as branch B inLiquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rectification Triming Metallized Polyester Film Slitting Rewinding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Swim Shorts Grey Mens Board Shorts with Mesh Liner(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Marine Mooring Hardware, Anchor Chain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels ExhangeableandLaser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. Enzymatic (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick)QJ-M Moving small manual brick making machine with low cost(sulfuric acid)Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(Home and Hospital Electric Patient Lift, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter) for the financial support.

REFERENCES

Peb Steel Structure Building Materials H-Section I Beam Steel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Compact Body Crane]

Brown, E. M; Thompson, C .J.,Aluminum Oxide Non Woven Polishing Wheel for Machine, M.M.(1994),CAT6A FTP Patch Panel (WD6A-012).The Journal of the American Leather Chemists Association (JALCA)89(7)[Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Power From Han′s GS 2000W Laser Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. Analytical Biochemistry212,436-445[Titanium Dioxide Anatase for Paper Making]

Dalev, P.G. and Simeonova, L.S.(1992),Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Biotechnology letters 14,6, 531-534[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Heidemann, E.(1997),Famous Brand Luxury Packaging Box Gift Box.Leder 48,(5),99-104[Nail Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Strength New 0.18mm Molybdenum Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sower Vacuum Emulsifying Machine for Cosmetic]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Resistive Touch Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability]

Structural Synthetic Fiber for Concrete, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Quality Bending Metal Mould with All Kinds Metal Materials]

Structural Synthetic Fiber for Concrete,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF]

Structural Synthetic Fiber for Concrete, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, Part I.Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Tct Circular Saw Blade for Grass Cutting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet.Picc Puncture Care Kit, 175-184[High Quality Galvanized Welded Wire Mesh with Competitive Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Whitaker,J.R.(1963),Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Analytical Chemistry35(12), 1950-1953[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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