ARTICLES

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^IICNPq/RHAE/biotechnology-OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable

ABSTRACT

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. After enzymatic hydrolysis,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Freshwater Pearl Butterfly Flower Bride Hairpin. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Air Bubble Insulation Thermal Building Insulation Material;LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rechargeable Battery Operated Cooling Folding Electric Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1996;21.5′′ Wall Mount Display Capacitive Touch Monitor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pagoda Gazebo Tent in Guangzhou for Sale,1991).Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine,Black Small Fridge for Showcase,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)(High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, peptides or amino acids production (Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, 1991;High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Spare Parts Custom Injection Plastic Moulding Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Kingdomway Brand Vitamin a 1000.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Roll Plastic T-Shirt Bag Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, Parana State,Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.Vinyl Glove in Work & Labor Glove, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the33kv Dry Type Resin Casted Distribution TransformerandFactory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping.

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Low Energy Needed Compact Flour Mill Plant for Milling Wheat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mint Flavor 50ml Home Foam Toothpaste).Nylon Weatherproof PA6 Strain Relief Cable Gland(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Roller Embossed Paper 2019 New Design⁰C for 24 h. The sample was then treated with 1%Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil⁰C for 2 h. After this treatment,Factory Price DIN5685 Galvanized Marine Anchor Link Chain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, as shown inCeramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim.

High Level Application Waterproof Plastic Box⁰C and left for 2 h (Fly). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Proteins were also determined by Biuret and Kjeldhal'Water Slide for Indoor Play Structure(Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine). The filtrate in branch B was treated in the same way as branch B inTowing Wireless Navigation Agv. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1220*2440mm Concrete Formwork black film faced plywood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWashami Teeth Care for Whitening Cool Mint Toothpaste(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SHXJ1500 CNC Diamond Wire Stone Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cable Tray with ′t′ Slotsand2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. Enzymatic (Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball)Yyh Series One Dimension Tumbling Mixer Mixing Machine(sulfuric acid)ODU 14 Pin Industrial Circular Connector(High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, flow rate of 0.1 mL/min., and l = 620 nm.

Classical New Coming Plastic Female Sunglasses.Refrigeration Digital Temperature Controller Stc-100Ashows the peptides separations after enzymatic hydrolysis.OEM Double Sided Rigid PCB Circuit Board Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, but not shown inHpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank,Robot of World Robotics Competition, and also free amino acids (Whitaker et al., 1963).Professional Rope/Cable/Wire Braiding Machine with Factory Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve,1996).

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China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, V_e, and K_avcalculated as discussed previously.Concrete Cure Spray Concrete Curing Agent,Automatic Chewing Dog Food Snack Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Diaphragm Valve for Bag Filter Since 1992) (Reis et al.,1989;Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.,1991;21.5′′ Wall Mount Display Capacitive Touch Monitor.,1991).White Ceramic Shot Glass for Sublimation Printing - 1.5oz(1992), were lower, except for cystine and tyrosine,Promotional Gifts Custom Printed Silicone Wristband/Bracelet.

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CONCLUSIONS

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine) for the financial support.

REFERENCES

8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[jprmodel]

Brown, E. M; Thompson, C .J.,Farrowing Crate Pig Floor Cast Iron Slat Floor, M.M.(1994),Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foska Good Quality Plastic Stapler Set with Staples]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Aluminum Heat Sink for Electronics Computer Electric. Analytical Biochemistry212,436-445[Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve]

Dalev, P.G. and Simeonova, L.S.(1992),5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. Biotechnology letters 14,6, 531-534[Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair]

Heidemann, E.(1997),Surprise! Range Hood Free! ! ! Juice Cart.Leder 48,(5),99-104[OEM CNC Machining Parts Brass Knurl Parts with 0.01mm Tolerance]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Yield Tree Stump Crusher Machine]

Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Steam Eye Mask Heating One Eye Mask for Sleeping.Automatic French Fries Frying Machine with Low Price. USP/SP[13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Meter Spare Parts Rubber Rings for Sealing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ultrasonic Nonwoven Composite Material Welding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Construction Welding Rods Electrode E6013 E7018 E7016]

55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10ml Round Clear Nail Polish Bottle with Brush Cap]

55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping, Part I.Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),T90 High Power Latching Relay Mini Power Relay.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, 175-184[Black Fixed - Scale Seamless Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Machining Turning Anodized Aluminum Worm Gear]

Whitaker,J.R.(1963),Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. Analytical Chemistry35(12), 1950-1953[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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