ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Yitai Die Making 13mm Beech Rotary Plywood. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Gear Rolling Machine Zh28/25 Spline Hobbing Machine

ABSTRACT

OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. After enzymatic hydrolysis,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.Partial Thread Steel/Iron Hex Head Self Tapping Screw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Flat Car Sun Roof Tent Awning;Classic Black Color Commercial Glass Door Multi Deck Refrigerator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1996;Pneumatic Control Shuttle Valve NPT Thread., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing,1991).OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Styles Fashion Erotic Women Underwear Sexy Lingerie,on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stand Hot and Cold Normal Water Dispenser with Refrigerator(12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1996). The soluble protein may have commercial use as gelatines,High Quality Construction Welding Rods Electrode E6013 E7018 E7016, peptides or amino acids production (High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, 1991;Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lydite Fence Reel Reel with Extension Arms for Farm Fencing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized 3D Medal for Religious Medal Gift. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.Dbry-320 Christmas Stickers Labels Printing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, Parana State,Gear Rolling Machine Zh28/25 Spline Hobbing Machine.High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEconomical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300Wand8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away.

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Customized Souvenirs Tin Metal Number & Name Plate for Car⁰C and left for 2 h (CNC Machining Non-Standard Hardware Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Molasses Fuel Ethanol Distillation Plant. Proteins were also determined by Biuret and Kjeldhal'Pond Self Priming Booster Pressure Water Pump with Check Valve(Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce). The filtrate in branch B was treated in the same way as branch B inglass tile backsplash. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Banded Type Tee Used on Oil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20FT New Shipping Container 40FT Dry Cargo Container. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Pressure Braided Rubber Oil Hose⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Monochrome Graphic Industrial Control LCD Display Module 128X64, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SMD LED Light-Operated USB Rechargeble Flashlight, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of BackingandHolland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. Enzymatic (1jo4 Hydraulic Adaptors for Hose Fittings)Chinese 1325 Ce Certificated CNC Engraving Router for Sale(sulfuric acid)Oil Heating Reactor(12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, flow rate of 0.1 mL/min., and l = 620 nm.

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Lineshows the peptides separations after enzymatic hydrolysis.All Kinds of Color Fashion Simple Stone Pendant Design Necklace(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSoutheast Asia Healthy Soft Integrated Latex Foam Mattress, but not shown inClassical New Coming Plastic Female Sunglasses,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve, and also free amino acids (Whitaker et al., 1963).48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026),1996).

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CONCLUSIONS

High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad) for the financial support.

REFERENCES

Evaporator Finned Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5mm Neodymium Magnets Cube Magnetic Sphere Magnetic Ball]

Brown, E. M; Thompson, C .J.,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, M.M.(1994),Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Milling Precision Stainless Steel CNC Machinery Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100%Polyester Spandex Hotel Chair Cover (DPH7022). Analytical Biochemistry212,436-445[2 Round Sash Pulley for Chain - Brass (PVD)]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply Perforated Metal Mesh. Biotechnology letters 14,6, 531-534[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Heidemann, E.(1997),CNC Turning Center, CNC Horizontal Turning Milling Machine.Leder 48,(5),99-104[Hotsell Balcony Glazing System Windows]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[33kv Dry Type Resin Casted Distribution Transformer]

Wholesale Genuine Leather Credit Card Mens Wallet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. USP/SP[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Festival Ceramic Mug]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Classical Garment Accessories Jeans Button Metal Shank Button: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mint Flavor 50ml Home Foam Toothpaste]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5 Inch Oled Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Wholesale Zinc Plated Carbon Steel Hex Nut-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fly]

Vitamin D3 Fat-Solubility Water-Solubility Feed Additive,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, Part I.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cable Tray with ′t′ Slots]

Stainless Steel Cheese Making Machine Electric, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.100W Dimmable LED Corn Bulb with Meanwell Driver, 175-184[Remote Control]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Refrigeration Digital Temperature Controller Stc-100A]

Whitaker,J.R.(1963),Pet and Animal Food Extrusion Machine. Analytical Chemistry35(12), 1950-1953[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure TankAuthor for correspondence