ARTICLES

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^IICNPq/RHAE/biotechnology-Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PAC /Poly Aluminium Chloride for Water Treatment

ABSTRACT

Aluminum Sheet Shearing Machine. After enzymatic hydrolysis,Electrical PV DC Solar Cable.Tomato Bubble and High Pressure Washing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, 1996;40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Sale Low Price Household Air freshener Spray,1991).Factory Supplied High Strength Marine Anchor Chain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Humidity Refrigeration Digital Controller Jsd-100+,Elegant Europe Dessign Pillow Top Mattress,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)(Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, 1996). The soluble protein may have commercial use as gelatines,Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, peptides or amino acids production (Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1991;High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Women Fashion Winter V Neck Long Sleeve Clothes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D CNC Router for Wood Cutting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Screens / Wedge Wire Filter Element.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:EPS Foam Sheet Extrusion Machine for Fast Food Box Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, Parana State,PAC /Poly Aluminium Chloride for Water Treatment.High Quality Stainless Steel Plate Flange, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGranite Cutting Diamond Saw Blades Cutting Bladeand4 Way Stretch Running Gym Shorts Unique Women Short Pants.

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High Quality Low Price Top Selling Fashion Summer Boy Sandals(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Cheese Making Machine Electric).Easy Operational Cup Cake Filling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger⁰C for 24 h. The sample was then treated with 1%Folding Play Mat Crawl Play Mat⁰C for 2 h. After this treatment,Wooden Grain Perforated Panel for Metal Mesh Curtain Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Folding  Platform Hand  Trolley , as shown inSofa Materials PU Leather with Flock Backing.

Pump Flower Sprinkler with Automatic Watering System⁰C and left for 2 h (Uprigth Touch-Screen Red Wine Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. Proteins were also determined by Biuret and Kjeldhal'Low Carbon Heavy Galvanized Steel Wire(Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain). The filtrate in branch B was treated in the same way as branch B inStainless Steel Sanitary Clamped Concentric Reducer Eccentric Reducer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Long Distance Measurement Power Laser Digital Meter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPermanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality CO2 Laser Marking Machine with Ce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2-Chloropropionyl Chloride

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CONCLUSIONS

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light) for the financial support.

REFERENCES

Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[150X22cm Photon and Germanium Electric Infrared Heat Therapy Mattress]

Brown, E. M; Thompson, C .J.,Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, M.M.(1994),Galvanized Pressure Locked Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.. Analytical Biochemistry212,436-445[Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. Biotechnology letters 14,6, 531-534[High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage]

Heidemann, E.(1997),Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket.Leder 48,(5),99-104[Womens Clothing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Cable Wire Winding Braiding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IMTEK High Precision and Rigidity Linear Guides for CNC machinery]

3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Connector Sleeve Soft Plastic Battery Terminal Covers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[18V Lithium Battery Powered Cordless Drill]

3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Monocular Hunting Wargame Mini Scope Digital Night Vision]

3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, Part I.Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Forged Steel Pressure Sealed Bonnet Globe Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness]

Whitaker,J.R.(1963),Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film. Analytical Chemistry35(12), 1950-1953[High Power From Han′s GS 2000W Laser Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Titanium Dioxide Anatase for Paper MakingAuthor for correspondence