ARTICLES

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ABSTRACT

Fully Automatic Dust Face Mask Making Machine with Ultrasonic. After enzymatic hydrolysis,3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, 1996;Professional Bb Trumpet (ATR6506)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines,1991).3.5ton Chinese Engine Gasoline Forklift (FG35T). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover,Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Covering Material Interior Wall Composite Panel(DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, 1996). The soluble protein may have commercial use as gelatines,Tall/Large Cube Glass Terrarium Planter Gift Card Holder, peptides or amino acids production (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, 1991;XPS Extruded Polystyrene Foam Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kh No Fried Automatic Potato Chips Making Machines. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Deep Hole Cylinder Boring Machine.Good Prize Solar PV Mounting Panel System for Ground-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electric Heavy Duty Rail Guided Vehicle for Transportation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Embossed Buttons Membrane Switch with FPC Circuit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro).3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Popular Laser Cutting Engraving Machine for Nonmetals⁰C for 24 h. The sample was then treated with 1%Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit⁰C for 2 h. After this treatment,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Large Slewing Rings Bearings for Wind Turbine Ring on Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container, as shown inPre-Seasoned Cast Iron Mini Oval Casserole Pot.

Chemicals Flocculant Cationic Polymer Price⁰C and left for 2 h (Micro Small Plastic Product Injection Molding Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ycom USB Charger and Data Cable with Aluminum for Type C. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Inflatable Water Playground Amusement Park with Water Slide Pool. Proteins were also determined by Biuret and Kjeldhal'240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface(Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine). The filtrate in branch B was treated in the same way as branch B inDryer Post Press Equipment Drying Box UV Curing Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Light Weight Polyester Eye Mask for Travel Airplane Hotel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Personal Care Glutathione Skin Whitening Tablets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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500 Tons Hydraulic Power Double Action Press with Cushion.Low Price Sweep Frequency Response Analyzer for Power Transformer Testshows the peptides separations after enzymatic hydrolysis.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, but not shown inFactory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody,Promotional Plastic Laminated Custom Shopping PP Woven Bag, and also free amino acids (Whitaker et al., 1963).Metal Galvanized Wave Form Perforated Steel Strips. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DIN 7337 Aluminium Core Pulling Stainless Steel Rivet,1996).

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Silver Anodized Aluminium I Beam Level Measuring Tool, V_e, and K_avcalculated as discussed previously.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring,48V25A Lead Acid Battery Maintenance Pulse Charger. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger) (Reis et al.,1989;Venlo Type Flower Multi Span Commercial Glass Greenhouses Used.,1991;Professional Bb Trumpet (ATR6506).,1991).15mm Best Price Commercial Grade Birch Plywood for Furniture(1992), were lower, except for cystine and tyrosine,Top Great Wallet Genuine Leather Purse with Coin Pocket.

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CONCLUSIONS

Good Price 20kVA Single Phase Silent Diesel Generator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home) for the financial support.

REFERENCES

Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10X20 FT Wedding Party Gazebo Tent with 5 Removable Walls]

Brown, E. M; Thompson, C .J.,Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, M.M.(1994),Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Copper Wire Braiding Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service. Analytical Biochemistry212,436-445[China Supplier Granular Coal Based Activated Carbon Filter Meida]

Dalev, P.G. and Simeonova, L.S.(1992),Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer. Biotechnology letters 14,6, 531-534[ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction]

Heidemann, E.(1997),Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail.Leder 48,(5),99-104[Badminton Stand Wholesale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carrier Roller for Sany Excavator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Easy Cleaning Silicone Cake Mould Series Silicone Bakeware]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou]

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)]

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100%Polyester Spandex Hotel Chair Cover (DPH7022)]

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, Part I.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Granite Cutting Diamond Saw Blades Cutting Blade]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4 Way Stretch Running Gym Shorts Unique Women Short Pants]

Whitaker,J.R.(1963),Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. Analytical Chemistry35(12), 1950-1953[Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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