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ABSTRACT

Laparoscopy Clip Applicator for Abdominal Surgery Uses. After enzymatic hydrolysis,High Yield Tree Stump Crusher Machine.Diaphragm Valve for Bag Filter Since 1992. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manufacturer Plastic Injection Mould Molding Nylon PP Injection, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, 1996;High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Forging Cast Metal Sand Die Casting,1991).ADC12 Aluminum Die Casting Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale,Custom Logo Cotton Terry Oversize Pullover Men Hoodie,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug(Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, 1996). The soluble protein may have commercial use as gelatines,Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, peptides or amino acids production (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 1991;PVC Fabric Rapid Roller Shutter for Clearn Room, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fiberglass Animal Poultry Insulation Grain Tower., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale 375ml Dark Green Glass Ice Wine Bottles.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder).Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop⁰C for 24 h. The sample was then treated with 1%Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved⁰C for 2 h. After this treatment,Single Screw Extruder for Plastic PP/PE Pipe Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, as shown inHeavy Industry Electric Transport Vehicle on Rails.

Gold Star Square Glass Candle Holder for Wedding Decoration⁰C and left for 2 h (CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.11kg 24L Low Pressure LPG Gas Cylinder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Proteins were also determined by Biuret and Kjeldhal'Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(Juice Doypack Packing Machine (ZLDB-4)). The filtrate in branch B was treated in the same way as branch B inGalvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite Color Enamel Bowls with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

API 5L Psl2/X65andSoutheast Asia Healthy Soft Integrated Latex Foam Mattress. Enzymatic (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad)Water Filter System Lamella Clarifier for River Water Treatment System(sulfuric acid)Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)(Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cabinet Shelf Support Pin Furniture Hardware Fittings, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cabinet Shelf Support Pin Furniture Hardware Fittings, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Slewing Gear for Tower Crane Slewing Winch Reducer) for the financial support.

REFERENCES

Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Concertina Razor Wire Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine, M.M.(1994),60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[48V25A Lead Acid Battery Maintenance Pulse Charger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Quartz Plate Optical Fused Clear Quartz Plate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solar Energy Storad Power Generation System. Analytical Biochemistry212,436-445[Customized 3D Medal for Religious Medal Gift]

Dalev, P.G. and Simeonova, L.S.(1992),110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). Biotechnology letters 14,6, 531-534[Stainless Steel Square Wire Mesh Netting with SGS]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Charms for Jewelry Making (Sold Per Bag 10PCS Inside)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Monocular Hunting Wargame Mini Scope Digital Night Vision: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic High Speed Folding & Gluing Machine for Corrugated Carton Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Embossing Roller for Tissue Paper]

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Liquid Spouted Stand up Pouch with Custom Logo]

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Wholesale Zinc Plated Carbon Steel Hex Nut]

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Italy Design Good Quality Hydraulic Motorcycle Lift Table, Part I.Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Double Sided Rigid PCB Circuit Board Manufacturer]

Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.Portable Telescopic Straw Metal Drinking Straw with Brush Case, 175-184[Excellent Yuchai Power Genset 800kw 100kVA Silent Electric Diesel Generator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan]

Whitaker,J.R.(1963),Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. Analytical Chemistry35(12), 1950-1953[High Density Rock Wool Wall Board with Thermal Insulation Performance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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