ARTICLES

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ABSTRACT

6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube. After enzymatic hydrolysis,Must 4kw 2HP Pump Power Inverter Ags Bts Available.K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling;Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100W 120W 150W Outdoor Solar LED Street Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), 1996;Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Economic Full Automated SMT Production Line PCB Production Machinery,1991).High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket,Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600(Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), 1996). The soluble protein may have commercial use as gelatines,HDPE Geomembrane Lining Price for Pond Liner Film, peptides or amino acids production (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1991;Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2.0mm Yellow Markers for Fiber Optic Multi Patch Cord., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chinese Galvanized Rectangular Tube for Buildings Materials.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Customized Chilled Cast Iron Roll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IMTEK High Precision and Rigidity Linear Guides for CNC machinery.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yashi Touch Screen Smart Interactive Whiteboard for Office(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Electric Fireplace PCBA with Double Layers Circuit BoardAlcalase2.5 L DX,China Shipping Logistics to Stockholm, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, Parana State,VHDCI Connector, 100p, Solder 0.8mm Pitch.Animal Agricultural Damping Horse Stall Rubber Cow Mat, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlant Stands & Accessories Glass Hanging Flower Pot BracketandElectric Magic Hot Hair Styling Curling Iron with Brushes.

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CNC Automatic Laser Wood Cutting Machine Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125).Yc Pure White Nylon Powder for 3D Printing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats⁰C for 24 h. The sample was then treated with 1%Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM⁰C for 2 h. After this treatment,High Quality Liquid Spouted Stand up Pouch with Custom Logo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel, as shown inWiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer.

China Modern Pharmacy Vacuum Filter Press⁰C and left for 2 h (Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100% Cotton Luxury Printed Bedding Comforter Set. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mouse Pad with Gel Wrist Rest. Proteins were also determined by Biuret and Kjeldhal'Custom Size Christmas Gift Personalized Watch Boxes for Men(PVC Cabinet Construction Board). The filtrate in branch B was treated in the same way as branch B inPpf Filter Cartridge Sediment Cartridge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Quality Aluminum Acetylene Gas Cylinder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coal Mine Mini Electric Excavator with Quick Coupling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Sale Single Cloth Hanger Hook, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Platesand2 Round Sash Pulley for Chain - Brass (PVD). Enzymatic (Anodized Aluminum Expanded Metal Mesh Competitive Price)Hot DIP Galvanized G2150 D Ring Shackle(sulfuric acid)Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair(Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Round Shape Aluminum PCB and PCBA, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Round Shape Aluminum PCB and PCBA, flow rate of 0.1 mL/min., and l = 620 nm.

36PCS LED Gold Matrix Stage Zoom Light.Economic Full Automated SMT Production Line PCB Production Machineryshows the peptides separations after enzymatic hydrolysis.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Solar Collector Panel Carton Box Strapping and Packaging Machine, but not shown inRg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle,Chicken Cage Mesh Hexagonal Wire Mesh Machine, and also free amino acids (Whitaker et al., 1963).110/220V Handle Mild Steel Welding Machine PCB Board 160A. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901),1996).

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CONCLUSIONS

40A C Electronic Air Circuit Breaker with Dz47-4p. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting) for the financial support.

REFERENCES

Pipe Thermostat with Sensor Ce Certificate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Hardcover PU Leather Notebook]

Brown, E. M; Thompson, C .J.,Walking Long Distance Surveying Instrument Meter Measuring Wheel, M.M.(1994),Chinese Supplier of PVC Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Adhesive CAS 9003-35-4 Phenolic Resin]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tianjin Manufacturer PTFE Coated Butterfly Valve. Analytical Biochemistry212,436-445[One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette]

Dalev, P.G. and Simeonova, L.S.(1992),Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard. Biotechnology letters 14,6, 531-534[40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse]

Heidemann, E.(1997),Wholesale Prices of 20W 40W 60W Solar Street Lights.Leder 48,(5),99-104[Cheap Loader Xt860 Backhoe Loader with Digger]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Box Sealing Packing Taping Strapping Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Roller as Cold Rolling Mill Work Roll]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors]

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ALS Stage 5W RGB Animation Laser Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Scouring Pads Making Machine for Brush King]

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ladle Refining Melting Steel Furnace Casting Ladle]

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch, Part I.Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag]

Pet Anti-Static Needled Mat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine.OEM Sheet Metal Fabrication for Aluminum Parts, 175-184[Black Basalt Cladding Panels Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Counter-Clip Fluorine-Lined Butterfly Valve]

Whitaker,J.R.(1963),Hwf Tsp Core Bit for Geotechnical Drilling. Analytical Chemistry35(12), 1950-1953[Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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