ARTICLES

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ABSTRACT

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel. After enzymatic hydrolysis,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cute Pink Cashmere Soft Plush Lining Warm Bootie, 1996;3kw 24V 310mm Vertical Agv Drive Wheel Assembly., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sofa Materials PU Leather with Flock Backing,1991).Three Phase 800mm Plate Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM Approved Popular Indoor Trampoline of Park,Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light(Cute Pink Cashmere Soft Plush Lining Warm Bootie, 1996). The soluble protein may have commercial use as gelatines,Wholesale Genuine Leather Credit Card Mens Wallet, peptides or amino acids production (Yitai Die Making 13mm Beech Rotary Plywood, 1991;Gear Rolling Machine Zh28/25 Spline Hobbing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Partial Thread Steel/Iron Hex Head Self Tapping Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cute Pink Cashmere Soft Plush Lining Warm Bootie, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flat Car Sun Roof Tent Awning.Classic Black Color Commercial Glass Door Multi Deck Refrigerator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stand Hot and Cold Normal Water Dispenser with Refrigerator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hexagonal Wire Mesh Chicken Wire Mesh High Quality).High Quality Construction Welding Rods Electrode E6013 E7018 E7016(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process⁰C for 24 h. The sample was then treated with 1%Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular⁰C for 2 h. After this treatment,Lydite Fence Reel Reel with Extension Arms for Farm Fencing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized 3D Medal for Religious Medal Gift, as shown in1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules⁰C and left for 2 h (Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dbry-320 Christmas Stickers Labels Printing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. Proteins were also determined by Biuret and Kjeldhal'Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor(All Kinds of Color Fashion Simple Stone Pendant Design Necklace). The filtrate in branch B was treated in the same way as branch B inFC to FC Single Mode Duplex Fiber Optic Metal Coupler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPetrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Bright Polished Mirror Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1jo4 Hydraulic Adaptors for Hose Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing) for the financial support.

REFERENCES

Chinese 1325 Ce Certificated CNC Engraving Router for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Durable EPDM Rubber Flooring Mat Gym Tiles (S-9006)]

Brown, E. M; Thompson, C .J.,Oil Heating Reactor, M.M.(1994),Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Liquid Spouted Stand up Pouch with Custom Logo]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Wholesale Zinc Plated Carbon Steel Hex Nut]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Analytical Biochemistry212,436-445[OEM Double Sided Rigid PCB Circuit Board Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),All Kinds of Color Fashion Simple Stone Pendant Design Necklace. Biotechnology letters 14,6, 531-534[Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan]

Heidemann, E.(1997),Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.Leder 48,(5),99-104[Thk Srs15]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Density Rock Wool Wall Board with Thermal Insulation Performance]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ALS Stage 5W RGB Animation Laser Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Di DIN3352 Double Orifice Automatic Air Release Valve Dn65]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Prepainted Galvanized Steel Sheet/Wrinkle PPGL /Ppcr/PPGI]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Animal Agricultural Damping Horse Stall Rubber Cow Mat]

Toilet Roll Tissue Wrapping Machine with Auto - Counting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50 Grams Ozone Producing Device for Makeup Toner Producing Plant]

Toilet Roll Tissue Wrapping Machine with Auto - Counting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D CNC Router for Wood Cutting Machine]

Toilet Roll Tissue Wrapping Machine with Auto - Counting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Orange Monofilament Fishing Tackle Nylon Fish Net, Part I.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck]

By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diameter 1300mm Lifting Electro Magnet for Cast Iron.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%, 175-184[Garage Door Panel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail]

Whitaker,J.R.(1963),Evaporator Finned Tube. Analytical Chemistry35(12), 1950-1953[Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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