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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor

ABSTRACT

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. After enzymatic hydrolysis,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad.Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope;High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Bright Polished Mirror Sheet, 1996;Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Motorcycle Reflector Injection Plastic Parts Mould Maker,1991).O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China,Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Souvenirs Tin Metal Number & Name Plate for Car(Aluminum Bright Polished Mirror Sheet, 1996). The soluble protein may have commercial use as gelatines,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, peptides or amino acids production (Molasses Fuel Ethanol Distillation Plant, 1991;Pond Self Priming Booster Pressure Water Pump with Check Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Banded Type Tee Used on Oil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20FT New Shipping Container 40FT Dry Cargo Container.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Bright Polished Mirror Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone.High Pressure Braided Rubber Oil Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Monochrome Graphic Industrial Control LCD Display Module 128X64(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Oil Heating Reactor, Parana State,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Bright Polished Mirror Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAhandLaser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System.

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6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood).All Kinds of Color Fashion Simple Stone Pendant Design Necklace(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve⁰C for 24 h. The sample was then treated with 1%48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System⁰C for 2 h. After this treatment,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Toilet Roll Tissue Wrapping Machine with Auto - Counting, as shown inPagoda Gazebo Tent in Guangzhou for Sale.

Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine⁰C and left for 2 h (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Orange Monofilament Fishing Tackle Nylon Fish Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). Proteins were also determined by Biuret and Kjeldhal'By6-8-200-L Automatic Doypack Premade Pouch Packing Machine(Stainless Steel Mechanic Parts for Lock Accessories with MIM Process). The filtrate in branch B was treated in the same way as branch B inHot Spinning Machine Set for Fire Extinguisher Cylinder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Diameter 1300mm Lifting Electro Magnet for Cast Iron.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEvaporator Finned Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100%Polyester Spandex Hotel Chair Cover (DPH7022), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply Perforated Metal Mesh, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, V_e, and K_avcalculated as discussed previously.Hexagonal Wire Mesh Chicken Wire Mesh High Quality,V-32 New Special UPS Solar Battery Cabinet Box Case. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats) (Reis et al.,1989;Molasses Fuel Ethanol Distillation Plant.,1991;Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).,1991).4-14 Manual Staple Gun with Staples for Hand Tools(1992), were lower, except for cystine and tyrosine,Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan.

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CONCLUSIONS

Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1jo4 Hydraulic Adaptors for Hose Fittings) for the financial support.

REFERENCES

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Excavator Undercarriage Replacement 198-32-31340 Track Bolt]

Brown, E. M; Thompson, C .J.,Biomass Drum Wood Chipper with Competitive Price, M.M.(1994),High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agriculture Film for Corn Silage Wrap Baler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Forged Steel Pressure Sealed Bonnet Globe Valve. Analytical Biochemistry212,436-445[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Biotechnology letters 14,6, 531-534[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Heidemann, E.(1997),Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.Leder 48,(5),99-104[APP Fireplace Remote Control Power Saving PCBA]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Dbry-320 Christmas Stickers Labels Printing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure. USP/SP[Gym Fitness Equipment Adjustable Weight Bench]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-80W All in One Outdoor LED Street Light Solar Lamp: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[alexandrite laser machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reusable Metal Stainless Steel Drinking Straws]

Hot Sale Safety Vinyl PVC Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Hot Sale Safety Vinyl PVC Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ D1400]

Hot Sale Safety Vinyl PVC Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Horizental Stainless Steel Food Grade Milk Tank for Milking, Part I.Multi Parameter Small Digital Domestic Water Meter TDS Meter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sanhe FC-2 Poultry Air Vent.Picosecond Laser Tattoo Removal Skin Care Medical Instrument, 175-184[1x32 Plc Splitter Sm Steel Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Pressure Fitting Quick Coupling Hydraulic Plug]

Whitaker,J.R.(1963),XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Analytical Chemistry35(12), 1950-1953[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hv / Coating Material Grade CMC Medium ViscosityAuthor for correspondence