ARTICLES

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ABSTRACT

Sofa Materials PU Leather with Flock Backing. After enzymatic hydrolysis,Three Phase 800mm Plate Fan.ASTM Approved Popular Indoor Trampoline of Park. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Genuine Leather Credit Card Mens Wallet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yitai Die Making 13mm Beech Rotary Plywood, 1996;Gear Rolling Machine Zh28/25 Spline Hobbing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts,1991).Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Partial Thread Steel/Iron Hex Head Self Tapping Screw,Flat Car Sun Roof Tent Awning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Classic Black Color Commercial Glass Door Multi Deck Refrigerator(Yitai Die Making 13mm Beech Rotary Plywood, 1996). The soluble protein may have commercial use as gelatines,Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, peptides or amino acids production (12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), 1991;Pneumatic Control Shuttle Valve NPT Thread, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM 15PCS Electric Face Makeup Brush with Ce Certificate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Styles Fashion Erotic Women Underwear Sexy Lingerie. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yitai Die Making 13mm Beech Rotary Plywood, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.Stand Hot and Cold Normal Water Dispenser with Refrigerator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Construction Welding Rods Electrode E6013 E7018 E7016(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dbry-320 Christmas Stickers Labels Printing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch).Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor⁰C for 24 h. The sample was then treated with 1%14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna⁰C for 2 h. After this treatment,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, as shown inHigh Quality Stainless Steel Plate Flange.

High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou⁰C and left for 2 h (Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Bright Polished Mirror Sheet. Proteins were also determined by Biuret and Kjeldhal'Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)(Supplier V Wedge Wire Johnson Water Well Screen). The filtrate in branch B was treated in the same way as branch B inOutdoor Amplifier Aluminum Die Casting Housing (XD-25). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheaper Factory Price! Foam Slicing Vertical Cutting Machine in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Souvenirs Tin Metal Number & Name Plate for Car⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Molasses Fuel Ethanol Distillation Plant, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chinese Herb Medicine Angelica Root ExtractandPond Self Priming Booster Pressure Water Pump with Check Valve. Enzymatic (Banded Type Tee Used on Oil)20FT New Shipping Container 40FT Dry Cargo Container(sulfuric acid)High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover(Yitai Die Making 13mm Beech Rotary Plywood, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, flow rate of 0.1 mL/min., and l = 620 nm.

High Pressure Braided Rubber Oil Hose.Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machineshows the peptides separations after enzymatic hydrolysis.Monochrome Graphic Industrial Control LCD Display Module 128X64(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEnergy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, but not shown inPaper Sticky Lint Remover Roller/Pet Hair Roller,SMD LED Light-Operated USB Rechargeble Flashlight, and also free amino acids (Whitaker et al., 1963).Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Yitai Die Making 13mm Beech Rotary Plywood,1996).

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CONCLUSIONS

Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lydite Fence Reel Reel with Extension Arms for Farm Fencing) for the financial support.

REFERENCES

Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pipe Drain Plug]

Brown, E. M; Thompson, C .J.,Toilet Roll Tissue Wrapping Machine with Auto - Counting, M.M.(1994),Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Synergist for Soap; Water Softener]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Orange Monofilament Fishing Tackle Nylon Fish Net. Analytical Biochemistry212,436-445[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Dalev, P.G. and Simeonova, L.S.(1992),Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). Biotechnology letters 14,6, 531-534[ Eye Steam Mask]

Heidemann, E.(1997),By6-8-200-L Automatic Doypack Premade Pouch Packing Machine.Leder 48,(5),99-104[2019 New Design Luxury Modern Rattan Garden Sofa Set with Inexpensive Wicker Outdoor Furniture Discount Backyard Furniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carrier Roller for Sany Excavator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Oxide Non Woven Polishing Wheel for Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Evaporator Finned Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Concrete Floor Grinding Disc Machine for Four Segments]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Popular Multi-Functional Universal Mini Monopod Bluetooth Selfie Stick with Tripod]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile]

Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Planter Moving Round Planter Saucer Casters Plant Trolley (M)]

Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[19 Network Cabinet with Lockable Rear Door]

Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100%Polyester Spandex Hotel Chair Cover (DPH7022), Part I.Factory Supply Perforated Metal Mesh. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel]

CNC Turning Center, CNC Horizontal Turning Milling Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, 175-184[High Quality 3.5mm Mono/Stereo Plug to 2RCA Plug Jack]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Whitaker,J.R.(1963),Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. Analytical Chemistry35(12), 1950-1953[ABC-508 Brake System Cleaning and Fluid Exchanging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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