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ABSTRACT

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Key words;SMD LED Light-Operated USB Rechargeble Flashlight;Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1jo4 Hydraulic Adaptors for Hose Fittings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chinese 1325 Ce Certificated CNC Engraving Router for Sale, 1996;Oil Heating Reactor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator,1991).6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,All Kinds of Color Fashion Simple Stone Pendant Design Necklace,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System(Chinese 1325 Ce Certificated CNC Engraving Router for Sale, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb, peptides or amino acids production (Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, 1991;Toilet Roll Tissue Wrapping Machine with Auto - Counting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Orange Monofilament Fishing Tackle Nylon Fish Net.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chinese 1325 Ce Certificated CNC Engraving Router for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰By6-8-200-L Automatic Doypack Premade Pouch Packing Machine.Diameter 1300mm Lifting Electro Magnet for Cast Iron-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Supply Perforated Metal Mesh, Parana State,High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.CNC Turning Center, CNC Horizontal Turning Milling Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chinese 1325 Ce Certificated CNC Engraving Router for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the API 5L Psl2/X65and5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover.

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Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap).Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Classical Garment Accessories Jeans Button Metal Shank Button⁰C for 24 h. The sample was then treated with 1%Vitamin D3 Fat-Solubility Water-Solubility Feed Additive⁰C for 2 h. After this treatment,Factory Wholesale Zinc Plated Carbon Steel Hex Nut. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V, as shown inPVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh.

Stainless Steel Cheese Making Machine Electric⁰C and left for 2 h (Hexagonal Wire Mesh Chicken Wire Mesh High Quality). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100W Dimmable LED Corn Bulb with Meanwell Driver. Proteins were also determined by Biuret and Kjeldhal'Pet and Animal Food Extrusion Machine(1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China). The filtrate in branch B was treated in the same way as branch B inwww.nsen-valve.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hexagonal Wire Mesh Chicken Wire Mesh High Quality. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avV-32 New Special UPS Solar Battery Cabinet Box Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.All Kinds of Color Fashion Simple Stone Pendant Design Necklaceshows the peptides separations after enzymatic hydrolysis.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, but not shown inColorful  Reflective Sticker Reflective Tape,80W All in One Outdoor LED Street Light Solar Lamp, and also free amino acids (Whitaker et al., 1963).Hot Sale Safety Vinyl PVC Flooring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chinese 1325 Ce Certificated CNC Engraving Router for Sale,1996).

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CONCLUSIONS

Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine) for the financial support.

REFERENCES

SUS316 Hexagon Socket Set Screws with Cone Point, DIN914, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6 Doors Commercial Refrigerator]

Brown, E. M; Thompson, C .J.,Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, M.M.(1994),Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor.The Journal of the American Leather Chemists Association (JALCA)89(7)[360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Embossing Roller for Tissue Paper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. Analytical Biochemistry212,436-445[High Quality Liquid Spouted Stand up Pouch with Custom Logo]

Dalev, P.G. and Simeonova, L.S.(1992),Explosion-Proof Housing Sealing Dispensing Machine. Biotechnology letters 14,6, 531-534[Factory Wholesale Zinc Plated Carbon Steel Hex Nut]

Heidemann, E.(1997),Tube Mills for Thin Wall Tubing.Leder 48,(5),99-104[Twin Screw Steam Floating Aqua Floating Fish Feed Pellet Extruder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Double Sided Rigid PCB Circuit Board Manufacturer]

Banded Type Tee Used on Oil, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core.Car Badge Custom Emblem Auto Logo Sign for Advertising. USP/SP[Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Density Rock Wool Wall Board with Thermal Insulation Performance]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White Color Enamel Bowls with High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Two Wheel Street Skateboard Factory X8 Roller Skatecycle Freerider Cycle Skate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ALS Stage 5W RGB Animation Laser Light]

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Di DIN3352 Double Orifice Automatic Air Release Valve Dn65]

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Animal Agricultural Damping Horse Stall Rubber Cow Mat]

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rear Wheel Exchangeable Aluminum Manual Wheelchair, Part I.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50 Grams Ozone Producing Device for Makeup Toner Producing Plant]

Graphic 128X128 Dots Cog LCD, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen.Tcn Sex Toy Vending Machines for Sale S800-10, 175-184[45W Pd Quick Charger 45W QC3.0 Smart Charger]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3D CNC Router for Wood Cutting Machine]

Whitaker,J.R.(1963),Silver 8 Inch Double Thick Round Cake Board. Analytical Chemistry35(12), 1950-1953[High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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