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ABSTRACT

High Pressure Braided Rubber Oil Hose. After enzymatic hydrolysis,Monochrome Graphic Industrial Control LCD Display Module 128X64.SMD LED Light-Operated USB Rechargeble Flashlight. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden;1jo4 Hydraulic Adaptors for Hose Fittings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chinese 1325 Ce Certificated CNC Engraving Router for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Oil Heating Reactor, 1996;Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,1991).All Kinds of Color Fashion Simple Stone Pendant Design Necklace. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve,48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb(Oil Heating Reactor, 1996). The soluble protein may have commercial use as gelatines,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, peptides or amino acids production (Toilet Roll Tissue Wrapping Machine with Auto - Counting, 1991;Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Orange Monofilament Fishing Tackle Nylon Fish Net., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Oil Heating Reactor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Diameter 1300mm Lifting Electro Magnet for Cast Iron.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Evaporator Finned Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cake/Cookies/Bread Automatic Package and Packing Machine).Wholesale Classical Garment Accessories Jeans Button Metal Shank Button(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vitamin D3 Fat-Solubility Water-Solubility Feed Additive⁰C for 24 h. The sample was then treated with 1%Factory Wholesale Zinc Plated Carbon Steel Hex Nut⁰C for 2 h. After this treatment,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Cheese Making Machine Electric, as shown inWire Cutting Clamping Beam for Used Sodick Wire EDM Machine.

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts⁰C and left for 2 h (Mini Car Portable Fridge 10L Mini Fridge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100W Dimmable LED Corn Bulb with Meanwell Driver. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet and Animal Food Extrusion Machine. Proteins were also determined by Biuret and Kjeldhal'Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product). The filtrate in branch B was treated in the same way as branch B inCustom Logo Stickers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hexagonal Wire Mesh Chicken Wire Mesh High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%V-32 New Special UPS Solar Battery Cabinet Box Case. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4-14 Manual Staple Gun with Staples for Hand Tools(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biomass Drum Wood Chipper with Competitive Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Sheet Shearing MachineandWood Grain Embossed Film for Flooringshow the gel chromatographic separation of soluble protein with Sephadex G-Horizental Stainless Steel Food Grade Milk Tank for Milking.Multi Parameter Small Digital Domestic Water Meter TDS Meter, and another peak at 45 mL not shown in Tree Shaving Machine, for peptides lower than 3000 Da.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display(Condell et al.,1993) .

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Sanhe FC-2 Poultry Air Vent, V_e, and K_avcalculated as discussed previously.Picosecond Laser Tattoo Removal Skin Care Medical Instrument,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Black Color Hexagon Bolt with High Quality) (Reis et al.,1989;Toilet Roll Tissue Wrapping Machine with Auto - Counting.,1991;Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator.,1991).Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849(1992), were lower, except for cystine and tyrosine,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart.

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CONCLUSIONS

SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100%Polyester Spandex Hotel Chair Cover (DPH7022)) for the financial support.

REFERENCES

Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Power IP65 Waterproof Outdoor 50W Solar LED Flood Light Price]

Brown, E. M; Thompson, C .J.,Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, M.M.(1994),Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Wok with Soft Touch Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Explosion-Proof Housing Sealing Dispensing Machine. Analytical Biochemistry212,436-445[Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Tube Mills for Thin Wall Tubing. Biotechnology letters 14,6, 531-534[Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Digital Printing Football Fans Flag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Crimped Wire Mesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light]

Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rear Wheel Exchangeable Aluminum Manual Wheelchair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle]

Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature]

Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, Part I.Graphic 128X128 Dots Cog LCD. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector]

Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tcn Sex Toy Vending Machines for Sale S800-10.Silver 8 Inch Double Thick Round Cake Board, 175-184[PVC Stretch Film for Making Mobile Phone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set]

Whitaker,J.R.(1963),High Precision Machining Auto Parts Metal Contact. Analytical Chemistry35(12), 1950-1953[Wholesale Black Galvanized Head Stainless Steel Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006Author for correspondence