ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Kh No Fried Automatic Potato Chips Making Machines

ABSTRACT

Deep Hole Cylinder Boring Machine. After enzymatic hydrolysis,Good Prize Solar PV Mounting Panel System for Ground.Electric Heavy Duty Rail Guided Vehicle for Transportation. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sower Vacuum Emulsifying Machine for Cosmetic;ASTM A240 309s Stainless Steel Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Car Mattress Back Inflatable Air Bed Car, 1996;24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine,1991).Single Embossed Buttons Membrane Switch with FPC Circuit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card,Popular Laser Cutting Engraving Machine for Nonmetals,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit(Car Mattress Back Inflatable Air Bed Car, 1996). The soluble protein may have commercial use as gelatines,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, peptides or amino acids production (Large Slewing Rings Bearings for Wind Turbine Ring on Sale, 1991;Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chemicals Flocculant Cationic Polymer Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ycom USB Charger and Data Cable with Aluminum for Type C.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Inflatable Water Playground Amusement Park with Water Slide Pool. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Car Mattress Back Inflatable Air Bed Car, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface.Light Weight Polyester Eye Mask for Travel Airplane Hotel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Personal Care Glutathione Skin Whitening Tablets, Parana State,Kh No Fried Automatic Potato Chips Making Machines.Customized Over Size Boys Hoodie with Embroidered, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Car Mattress Back Inflatable Air Bed Car, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Speed Carton Folding and Gluing Machine Box Sealing and Strapping MachineandBest Selling Counter Balance Forklift 3 Ton LPG Forklift Truck.

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Digital Printing Football Fans Flag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe).Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine⁰C for 24 h. The sample was then treated with 1%PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal⁰C for 2 h. After this treatment,500 Tons Hydraulic Power Double Action Press with Cushion. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional Plastic Laminated Custom Shopping PP Woven Bag, as shown inSinicline Custom Brand with Plastic Pouch Hangtag for Hats.

Metal Galvanized Wave Form Perforated Steel Strips⁰C and left for 2 h (Hot Forming Pushing Elbow Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. Proteins were also determined by Biuret and Kjeldhal'Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale(Greenhouse Fixing Film Profile Lock Channel and Spring Wire). The filtrate in branch B was treated in the same way as branch B inEU Wall Switch Socket Mobile Socket USB Travel Adaptor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Silver Anodized Aluminium I Beam Level Measuring Tool.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av48V25A Lead Acid Battery Maintenance Pulse Charger(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Great Wallet Genuine Leather Purse with Coin Pocket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price 20kVA Single Phase Silent Diesel Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book) for the financial support.

REFERENCES

Advanced Unlocking Tool for Locksmith, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Basketball Surface Rubber Tiles Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Screen Printing Materials and Equipments Screen Printing Frames, M.M.(1994),China Supplier Hard Ware Fittings Gas Spring for Tool Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. Analytical Biochemistry212,436-445[S Series China Generator Helical-Worm Crushers Reduction Box]

Dalev, P.G. and Simeonova, L.S.(1992),Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. Biotechnology letters 14,6, 531-534[Pre-Seasoned Cast Iron Baking Stone Cookware]

Heidemann, E.(1997),New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.Leder 48,(5),99-104[Stainless Steel Precision Machinery CNC Parts in CD4/316ss/Titanium]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855.CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc. USP/SP[Concrete Spare Part Electrical System Central Distribution Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[No. 1062 20PCS Tools]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reseller Ball Stud Fasteners]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Genuine Leather Credit Card Mens Wallet]

Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),AISI 201/202/301/304 Stainless Steel Welded Square Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate]

Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, Part I.Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle.Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, 175-184[Diffuse Photoelectric Sensor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

Whitaker,J.R.(1963),High Speed Copper Wire Braiding Making Machine. Analytical Chemistry35(12), 1950-1953[79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)Author for correspondence