ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Black Phosphated Bugle Head Csk Head Drywall Screw. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine

ABSTRACT

China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. After enzymatic hydrolysis,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence.6 Inch Plastic Breathing Cap for The IBC Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability;Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 1996;Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars,1991).Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Freshwater Pearl Butterfly Flower Bride Hairpin,Air Bubble Insulation Thermal Building Insulation Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh(OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 1996). The soluble protein may have commercial use as gelatines,Rechargeable Battery Operated Cooling Folding Electric Fan, peptides or amino acids production (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1991;21.5′′ Wall Mount Display Capacitive Touch Monitor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pagoda Gazebo Tent in Guangzhou for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Small Fridge for Showcase.Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Zircon Butterfly Open Ring Diamond Fashion Woemn RingsAlcalase2.5 L DX,High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Spare Parts Custom Injection Plastic Moulding Manufacturer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, Parana State,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine.Kingdomway Brand Vitamin a 1000, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCarrier Roller for Sany ExcavatorandHot DIP Galvanized G2150 D Ring Shackle.

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Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Concrete Floor Grinding Disc Machine for Four Segments).High Speed Roll Plastic T-Shirt Bag Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine⁰C for 24 h. The sample was then treated with 1%Wholesale Black Galvanized Head Stainless Steel Screw⁰C for 2 h. After this treatment,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)45*60cm Anti-Fatigue ESD Rubber Floor Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, as shown inDark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile.

Vinyl Glove in Work & Labor Glove⁰C and left for 2 h (Planter Moving Round Planter Saucer Casters Plant Trolley (M)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Energy Needed Compact Flour Mill Plant for Milling Wheat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nylon Weatherproof PA6 Strain Relief Cable Gland. Proteins were also determined by Biuret and Kjeldhal'Steel Roller Embossed Paper 2019 New Design(19 Network Cabinet with Lockable Rear Door). The filtrate in branch B was treated in the same way as branch B inm.sincere-paper.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Price DIN5685 Galvanized Marine Anchor Link Chain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Level Application Waterproof Plastic Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar PanelandWater Slide for Indoor Play Structure. Enzymatic (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder)1220*2440mm Concrete Formwork black film faced plywood(sulfuric acid)Washami Teeth Care for Whitening Cool Mint Toothpaste(OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, flow rate of 0.1 mL/min., and l = 620 nm.

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300Wshows the peptides separations after enzymatic hydrolysis.SHXJ1500 CNC Diamond Wire Stone Cutting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inABC-508 Brake System Cleaning and Fluid Exchanging Machine, but not shown inBasic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388),Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, and also free amino acids (Whitaker et al., 1963).2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part,1996).

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CAS 9067-32-7 Hyaluronic Acid/ Sodium HyaluronateandFactory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motorshow the gel chromatographic separation of soluble protein with Sephadex G-Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Yyh Series One Dimension Tumbling Mixer Mixing Machine, and another peak at 45 mL not shown inMetal Separating Gold Brass Zippers for Sale, for peptides lower than 3000 Da.ODU 14 Pin Industrial Circular Connector(Condell et al.,1993) .

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Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, V_e, and K_avcalculated as discussed previously.Classical New Coming Plastic Female Sunglasses,OEM Double Sided Rigid PCB Circuit Board Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts) (Reis et al.,1989;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.,1991;Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.,1991).Robot of World Robotics Competition(1992), were lower, except for cystine and tyrosine,Professional Rope/Cable/Wire Braiding Machine with Factory Price.

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CONCLUSIONS

Aluminum PCB Circuit Board SMD PCBA LED PCB. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Spare Parts Custom Injection Plastic Moulding Manufacturer) for the financial support.

REFERENCES

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Copy Paper Writing Paper Newspaper A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Brown, E. M; Thompson, C .J.,Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, M.M.(1994),China Supplier Steel Machine Bellows Cover Telescopic Shield Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Concrete Cure Spray Concrete Curing Agent. Analytical Biochemistry212,436-445[Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Chewing Dog Food Snack Machine. Biotechnology letters 14,6, 531-534[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

Heidemann, E.(1997),White Ceramic Shot Glass for Sublimation Printing - 1.5oz.Leder 48,(5),99-104[m.wh-machine.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

Powder/Spices Powder Packing Machine with Four/Three Side Seal, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Promotional Gifts Custom Printed Silicone Wristband/Bracelet.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. USP/SP[Flat Pack Temperary Container House 20FT 40FT]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Engieered Veneer Faced Commericial Plywood]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[wet drill bit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Farrowing Crate Pig Floor Cast Iron Slat Floor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

Farrowing Crate Pig Floor Cast Iron Slat Floor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Positive Drive Turn Belt for Spiral Freezing Equipment]

Farrowing Crate Pig Floor Cast Iron Slat Floor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Aluminum Heat Sink for Electronics Computer Electric, Part I.5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yitai Die Making 13mm Beech Rotary Plywood]

Surprise! Range Hood Free! ! ! Juice Cart, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steam Eye Mask Heating One Eye Mask for Sleeping.Automatic French Fries Frying Machine with Low Price, 175-184[Portable Metal Laser Printing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Whitaker,J.R.(1963),Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns. Analytical Chemistry35(12), 1950-1953[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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