ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Galvanized Roller Shutter Window Torsion Springs. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale

ABSTRACT

Carbon Graphite Crucible. After enzymatic hydrolysis,Decorative Aluminum Solid Panel for Commercial Building.Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Di DIN3352 Double Orifice Automatic Air Release Valve Dn65;Magic Hair Twist   Curl Sponge Brush; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Geyi Disposable Laparoscopic Visible Optical Trocar, 1996;Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bimetallic Thermometer Industrial Temperature Gauge,1991).19 Network Cabinet with Lockable Rear Door. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery,175mm Diamond Cutting Blade for Granite&Tiles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables(Geyi Disposable Laparoscopic Visible Optical Trocar, 1996). The soluble protein may have commercial use as gelatines,PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, peptides or amino acids production (Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, 1991;Uprigth Touch-Screen Red Wine Chiller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Geyi Disposable Laparoscopic Visible Optical Trocar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard.Groove Type Wood Log Tree Peeling and Debarking Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality CE Digital Display Adjustable Power Supply(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor).Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Tensile Strength Galvanized Steel Wire⁰C for 24 h. The sample was then treated with 1%24 Volt Fire Alarm Strobe Light Horn Strobe Sounder⁰C for 2 h. After this treatment,from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Price PVC Coated Fiberglass Mesh Insect Window Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, as shown inCNC Machining Turning Anodized Aluminum Worm Gear.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Non-Expensive Cracking Agent) for the financial support.

REFERENCES

Pneumatic Control Shuttle Valve NPT Thread, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Superalloy]

Brown, E. M; Thompson, C .J.,Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, M.M.(1994),OEM 15PCS Electric Face Makeup Brush with Ce Certificate.The Journal of the American Leather Chemists Association (JALCA)89(7)[35 Degree Escalator with Auto Start Function in Parallel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CH Series Pid Intelligent Temperature Controller CH402]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Styles Fashion Erotic Women Underwear Sexy Lingerie. Analytical Biochemistry212,436-445[Fashion Metal Button Crystal Shank Button for Garment]

Dalev, P.G. and Simeonova, L.S.(1992),on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. Biotechnology letters 14,6, 531-534[High-End Business Magnet Leather Ring Binder Notebook]

Heidemann, E.(1997),Stand Hot and Cold Normal Water Dispenser with Refrigerator.Leder 48,(5),99-104[Safe Lock]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Eye Grab Bend Hook]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chinese Galvanized Rectangular Tube for Buildings Materials]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[FSTN LCD Graphic Module Cog White Characters on Blue Background FPC Module]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[UF Membrane Aqu160 with Competitive Price and High Quality]

Lydite Fence Reel Reel with Extension Arms for Farm Fencing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC Connector Sleeve Soft Plastic Battery Terminal Covers]

Lydite Fence Reel Reel with Extension Arms for Farm Fencing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price DIN5685 Galvanized Marine Anchor Link Chain]

Lydite Fence Reel Reel with Extension Arms for Farm Fencing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized 3D Medal for Religious Medal Gift, Part I.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)]

Dbry-320 Christmas Stickers Labels Printing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor.Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, 175-184[Buildings of Steel Structure for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Steel Machine Bellows Cover Telescopic Shield Cover]

Whitaker,J.R.(1963),14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Analytical Chemistry35(12), 1950-1953[Factory Supply Perforated Metal Mesh]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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