ARTICLES

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ABSTRACT

Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). After enzymatic hydrolysis,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen.High Tensile Strength Galvanized Steel Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Price PVC Coated Fiberglass Mesh Insect Window Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1996;Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory OEM CNC Machined Gear Motor Shaft,1991).High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pink French Loop Terry Knit Fabric for Jacket,Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts(Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1996). The soluble protein may have commercial use as gelatines,500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, peptides or amino acids production (Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, 1991;Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cute Pink Cashmere Soft Plush Lining Warm Bootie.3kw 24V 310mm Vertical Agv Drive Wheel Assembly-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sofa Materials PU Leather with Flock Backing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Genuine Leather Credit Card Mens Wallet, Parana State,Custom Striped Compression Soccer Socks for Adults.Yitai Die Making 13mm Beech Rotary Plywood, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8oz 250ml Disposable Paper Soup Bowl with Lid for Take Awayand14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.

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Gear Rolling Machine Zh28/25 Spline Hobbing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap).OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale⁰C for 24 h. The sample was then treated with 1%Partial Thread Steel/Iron Hex Head Self Tapping Screw⁰C for 2 h. After this treatment,Flat Car Sun Roof Tent Awning. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Classic Black Color Commercial Glass Door Multi Deck Refrigerator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, as shown inCNC Machining Non-Standard Hardware Parts.

12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)⁰C and left for 2 h (Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pneumatic Control Shuttle Valve NPT Thread. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. Proteins were also determined by Biuret and Kjeldhal'OEM 15PCS Electric Face Makeup Brush with Ce Certificate(Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing). The filtrate in branch B was treated in the same way as branch B inXCMG Official Manufacturer Xe370ca China Hydraulic Crawler Excavator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Styles Fashion Erotic Women Underwear Sexy Lingerie.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStand Hot and Cold Normal Water Dispenser with Refrigerator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lydite Fence Reel Reel with Extension Arms for Farm Fencing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Lineand1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Enzymatic (Customized 3D Medal for Religious Medal Gift)Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules(sulfuric acid)Dbry-320 Christmas Stickers Labels Printing Machine(Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.Southeast Asia Healthy Soft Integrated Latex Foam Mattressshows the peptides separations after enzymatic hydrolysis.14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inClassical New Coming Plastic Female Sunglasses, but not shown inSx Sm Fiber Optic MPO to MPO Adapter with Flange Type,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad, and also free amino acids (Whitaker et al., 1963).Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application,1996).

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Aluminum Bright Polished Mirror Sheet, V_e, and K_avcalculated as discussed previously.Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open),Professional Motorcycle Reflector Injection Plastic Parts Mould Maker. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture) (Reis et al.,1989;Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.,1991;Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor.,1991).O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W(1992), were lower, except for cystine and tyrosine,Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.

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CONCLUSIONS

Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building) for the financial support.

REFERENCES

Customized Souvenirs Tin Metal Number & Name Plate for Car, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[dx-blast.com]

Brown, E. M; Thompson, C .J.,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, M.M.(1994),Molasses Fuel Ethanol Distillation Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2 Round Sash Pulley for Chain - Brass (PVD)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pond Self Priming Booster Pressure Water Pump with Check Valve. Analytical Biochemistry212,436-445[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Dalev, P.G. and Simeonova, L.S.(1992),Banded Type Tee Used on Oil. Biotechnology letters 14,6, 531-534[33kv Dry Type Resin Casted Distribution Transformer]

Heidemann, E.(1997),20FT New Shipping Container 40FT Dry Cargo Container.Leder 48,(5),99-104[Alpha Fetoprotein Test Kit Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. USP/SP[Festival Ceramic Mug]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mint Flavor 50ml Home Foam Toothpaste]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Pressure Braided Rubber Oil Hose: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[700ml Custom Made Wine Glass Bottles for Vodka]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fly]

Monochrome Graphic Industrial Control LCD Display Module 128X64, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SMD LED Light-Operated USB Rechargeble Flashlight-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

Monochrome Graphic Industrial Control LCD Display Module 128X64,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cable Tray with ′t′ Slots]

Monochrome Graphic Industrial Control LCD Display Module 128X64, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, Part I.1jo4 Hydraulic Adaptors for Hose Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Refrigeration Digital Temperature Controller Stc-100A]

Chinese 1325 Ce Certificated CNC Engraving Router for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Oil Heating Reactor.Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 175-184[Hydraulic cutting steel truss c purlin roll forming machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Whitaker,J.R.(1963),6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Analytical Chemistry35(12), 1950-1953[Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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