ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Reusable Metal Stainless Steel Drinking Straws

ABSTRACT

Designed Steel Frame Factory Building. After enzymatic hydrolysis,Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Scouring Pads Making Machine for Brush King;Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Mix Stone Mosaic Tiles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1996;3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net,1991).Used/Second Hand 120mpm Continous Galvanizing Line/Cgi. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie,Racing Preformance PTFE Teflon Alloy Fuel Adapter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170(Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1996). The soluble protein may have commercial use as gelatines,High Quality Stainless Steel Expanded Metal Mesh, peptides or amino acids production (Popular Lovely Animal Design Inflatable Slide for Kids, 1991;Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hairs Comb for Pet Dog Cats.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.Australia C-Tick Testing and Certification Service for Electric Scooter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

35 Degree Escalator with Auto Start Function in ParallelAlcalase2.5 L DX,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Roller Wedge Screen Cylinder(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, Parana State,Reusable Metal Stainless Steel Drinking Straws.Push Pull Small Cable to Cable Connector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGsjl 91/1 Multibar Computerized Jacquard Warp Knitting MachineandTire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility.

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Stainless Steel Seamless Pipe ASTM A312 TP304 316(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM A240 309s Stainless Steel Sheet).Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars⁰C for 24 h. The sample was then treated with 1%Big Chocolate Storage Holding Tank, Chocolate Buffer Tank⁰C for 2 h. After this treatment,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Asco 353 Series 3/4 Pulse Valve Solenoid Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, as shown inIridium Power Denso Spark Plug Bk6rei-11 for Honda Cars.

Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle⁰C and left for 2 h (Chinese 1325 Ce Certificated CNC Engraving Router for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Purple UL 1095 16AWG Electronic Lead Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brushed Anodizing Aluminum Extrusion Profile for LED. Proteins were also determined by Biuret and Kjeldhal'PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps( Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights). The filtrate in branch B was treated in the same way as branch B inHot Oil Boiler Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,S235jo S235jr Steel Pipe Tube Shs Rhs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPre-Seasoned Cast Iron Mini Oval Casserole Pot(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Garden Save Energy with Solar Flame Wall Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog LeashandCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). Enzymatic (Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag)Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length(sulfuric acid)Non Stick Silicone Coated Fiberglass Cloth(Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, flow rate of 0.1 mL/min., and l = 620 nm.

Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius.Custom Apparel Applique Round Patch School Club Embroidery Collar Patchshows the peptides separations after enzymatic hydrolysis.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, but not shown inDC Brushless Motor for CNC Machine with ISO9001,DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, and also free amino acids (Whitaker et al., 1963).3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine,1996).

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Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, V_e, and K_avcalculated as discussed previously.Laparoscopy Clip Applicator for Abdominal Surgery Uses,High Yield Tree Stump Crusher Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light) (Reis et al.,1989;Popular Lovely Animal Design Inflatable Slide for Kids.,1991;3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank.,1991).Diaphragm Valve for Bag Filter Since 1992(1992), were lower, except for cystine and tyrosine,4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf.

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CONCLUSIONS

Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h) for the financial support.

REFERENCES

Manufacturer Plastic Injection Mould Molding Nylon PP Injection, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Vape Pen 1.4ml Capacity Multi Flavour Disposable Pod Device]

Brown, E. M; Thompson, C .J.,Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, M.M.(1994),High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Shipping Logistics to Stockholm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Forging Cast Metal Sand Die Casting. Analytical Biochemistry212,436-445[800W Electric Motor Price Lawn Mower Motor Concrete Mixer]

Dalev, P.G. and Simeonova, L.S.(1992),ADC12 Aluminum Die Casting Parts. Biotechnology letters 14,6, 531-534[Compression Polyester Light Ankle Support Brace for Youth]

Heidemann, E.(1997),Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale.Leder 48,(5),99-104[5 in 1 38X38cm Textile Press Hot Press 1450W Rotation 360 Degrees]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube]

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Logo Cotton Terry Oversize Pullover Men Hoodie.Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug. USP/SP[Road Construction Fabric Non-Woven Geotextile 200GSM]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Geyi Disposable Laparoscopic Visible Optical Trocar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Advanced Unlocking Tool for Locksmith]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Binocular Loupes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China]

Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Fabric Rapid Roller Shutter for Clearn Room-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Great Wallet Genuine Leather Purse with Coin Pocket]

Seikofire S-66 Palm OTDR Sm/mm Fiber Testing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header]

Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiberglass Animal Poultry Insulation Grain Tower, Part I.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Sheet Metal Fabrication for Aluminum Parts]

Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale 375ml Dark Green Glass Ice Wine Bottles.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, 175-184[Uhf Rfid Tag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Used/Second Hand 120mpm Continous Galvanizing Line/Cgi]

Whitaker,J.R.(1963),Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. Analytical Chemistry35(12), 1950-1953[Cabinet Shelf Support Pin Furniture Hardware Fittings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage TreatmentAuthor for correspondence