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ABSTRACT

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. After enzymatic hydrolysis,Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator.PVC Cabinet Construction Board, White 18mm PVC Foam Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Prefab Steel Building for Garment Factory, 1996;Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cable Tray with ′t′ Slots,1991).10 Gauge Galvanized Welded Wire Mesh for Construction Using. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Factory Inline Hockey Skate Puck,0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet(China Prefab Steel Building for Garment Factory, 1996). The soluble protein may have commercial use as gelatines,Disposable PS Foam Fast Food Box Making Machine, peptides or amino acids production (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), 1991;Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Foska Good Quality Plastic Stapler Set with Staples., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hotel SPA Swimming Pool, Sport Room, Bathroom Towel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Prefab Steel Building for Garment Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Powerful Cleaning Colorful Scouring Pad for Kitchen Use.500t Plywood Hot Press Woodworking Machine (12-25 layers)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10ml Round Clear Nail Polish Bottle with Brush Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDC 12V Power Relay for Smart House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Human Body Thermometer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., pH 3.25., mobile phase B: buffer solution of 0.01 mol/Greenhouse Fixing Film Profile Lock Channel and Spring Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine) for the financial support.

REFERENCES

Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Newly Reversible Sequin Wine Bottle Pouch Bottle Bag for Sublimation]

Brown, E. M; Thompson, C .J.,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, M.M.(1994),Hanwha SMT Chip Shooter LED Pick and Place Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cake/Cookies/Bread Automatic Package and Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ACP Aluminium Cmposite Panel Cladding Extrusions. Analytical Biochemistry212,436-445[Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine]

Dalev, P.G. and Simeonova, L.S.(1992),New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. Biotechnology letters 14,6, 531-534[Mini Car Portable Fridge 10L Mini Fridge]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Swim Shorts Grey Mens Board Shorts with Mesh Liner: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pink French Loop Terry Knit Fabric for Jacket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Plastic Pipe Price List and Fittings U-PVC Plumbing Conduit Pipes for Water Supply and Drainage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Omni Newest Manager Desk Office Smart Office Table Office Staff Table]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Tree Shaving Machine]

Whitaker,J.R.(1963),Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Analytical Chemistry35(12), 1950-1953[Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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