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ABSTRACT

Sweat Suit for Women Three Piece Tracksuit. After enzymatic hydrolysis,Cold Rolled Stainless Steel Strip /Coils 410/430/409.FRP Water Tank GRP Fishing Tanks From Factory Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Decorative House Stone Coated Metal Roof Tile;Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 1996;Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use,1991).Blue Steel Packing Strip. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Powder and Wax Brush with Wooden Handle,Cheap Price Paper Straws Individually Wrapped Drinking Straw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality(Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, peptides or amino acids production (Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose, 1991;Smelting and Heating Induction Machine (GW-HY187), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Home Products 16′ Perforated Flex Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1220 Fork Length 3-Wheel Electric Forklift Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits.FM USB Radio Audio Power Amplifier for Home Bar Club-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Union Metal Steel Building and Construction Material, Parana State,Flat Pack Temperary Container House 20FT 40FT.Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFully Automatic Dust Face Mask Making Machine with Ultrasonicand Locking Hinge Pin.

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450ml Sublimation Stainless Steel Milk Bottle (Colorful)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting).Lower Noise Colled Rolled Ball Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls⁰C for 24 h. The sample was then treated with 1%3.0kw Spindle CNC Router with Certificate for Woodworking⁰C for 2 h. After this treatment,800W Electric Motor Price Lawn Mower Motor Concrete Mixer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Cooled Split Type Ducted Air Conditioner AC Unit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric, as shown inPermanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets.

High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet⁰C and left for 2 h (Kingdomway Brand Vitamin a 1000). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gym Fitness Equipment Adjustable Weight Bench. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Sell P7.8 Transparent LED Display Screen. Proteins were also determined by Biuret and Kjeldhal'Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)(Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine). The filtrate in branch B was treated in the same way as branch B inNylon Back to Back Continuous Hook and Loop Wrap Tape Strap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Original Weichai Engine Truck Spare Parts Valve Cap 61500050105.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCeramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fused Quartz Powder 325mesh 500mesh 1250mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Various Types of Plastic Hooks Mould From Manufacturers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Adhesive Powder for Skim Coat/Wall Putty HPMCand10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower. Enzymatic (Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift)Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India(sulfuric acid)Soft Baby Suites Dinosaure Pijamas Kigurumi(Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply, flow rate of 0.1 mL/min., and l = 620 nm.

High Density Celuca Kitchen Cabinets PVC Foam Board.Automatic Cashew Processing Cashew Nuts Shelling Peeling Machineshows the peptides separations after enzymatic hydrolysis.2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Dipped Galvanized Oval Wire Brazil, but not shown inHans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric,E171 Standrad Titanium Dioxide Food Grade A200, and also free amino acids (Whitaker et al., 1963).Elbow Forming Machine Gorelocker HVAC Duct Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System,1996).

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460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, V_e, and K_avcalculated as discussed previously.304 Stainless Steel Anti-Corrosion Welded Wire Mesh,Custom Made Colorful Sublimation Womens Yoga Pants Wholesale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Seamless Pipe ASTM A312 TP304 316) (Reis et al.,1989;Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose.,1991;Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.,1991).Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter(1992), were lower, except for cystine and tyrosine,Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel.

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CONCLUSIONS

New Fashion PVC Sequins Adjustable Women Brace Suspenders. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (700 mm RP HP UHP Graphite Electrode Sold at Factory Price) for the financial support.

REFERENCES

Solid Flex-Drain Pipe Length 40′, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shenzhen CNC Milling Machining Manufacturer Custom Metal Box Voltage/VGA/Audio/Video Converters/HDD Digital Electrical Aluminum Housing/Casing/Enclosure]

Brown, E. M; Thompson, C .J.,ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator, M.M.(1994),in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case.The Journal of the American Leather Chemists Association (JALCA)89(7)[Offshore Drilling Platform Air Winch for Marine Oilfields]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet and Animal Food Extrusion Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500. Analytical Biochemistry212,436-445[High Level Application Waterproof Plastic Box]

Dalev, P.G. and Simeonova, L.S.(1992),W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/. Biotechnology letters 14,6, 531-534[Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker]

Heidemann, E.(1997),Cake/Cookies/Bread Automatic Package and Packing Machine.Leder 48,(5),99-104[Custom Logo Desk Flag Mini Feather Flag Teardrop Table Flag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[QJ-M Moving small manual brick making machine with low cost]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Pressure Locked Grating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Biobase Electrophoresis Lab Equipment Et-V2 Vertical Electrophoresis Tank]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck]

Fruit Plum Cleaning Washing Machine with Brushes Spray, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Various Types of Plastic Hooks Mould From Manufacturers]

Fruit Plum Cleaning Washing Machine with Brushes Spray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie]

Fruit Plum Cleaning Washing Machine with Brushes Spray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, Part I.Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord]

Jh21 Series Pneumatic Power Press CNC Punching Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Coal Fried Steam Boiler for Industry with Double Drum.Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, 175-184[OEM ODM Custom Made Metal Brass Parts CNC Machining Industrial Product]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass]

Whitaker,J.R.(1963),Stainless Steel Filter Strainer. Analytical Chemistry35(12), 1950-1953[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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