ARTICLES

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ABSTRACT

Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. After enzymatic hydrolysis,Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup.Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manual Foot Pedal Hydraulic Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, 1996;Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CH Series Pid Intelligent Temperature Controller CH402,1991).China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Precision Infusion Set with Filter and Needle for Single Use,Low Price Sweep Frequency Response Analyzer for Power Transformer Test,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, peptides or amino acids production (High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT, 1991;Mining Machinery Sand Vibrating Sieve for River Sand, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PRO Audio Loudspeaker PA Speaker System Line Array Speaker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Palet Coil Nail Welding Machine/Nail Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.High Quality MPO Singlemode Duplex Fiber Optic Adapter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hotel Bedding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)

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Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (As568 Standard Brown FKM Rubber O Ring for Sealing).Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Outlet Automatic Grain Tower Dryer Drying Equipment⁰C for 24 h. The sample was then treated with 1%Mesh Bag Packing 20kg Pure White Garlic⁰C for 2 h. After this treatment,Supreme 524 Mv Sea Kayak. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Trunk Organizer Foldable Water Proof's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), as shown inHigh Speed Roll Plastic T-Shirt Bag Making Machine.

Diesel Generator Parts AVR Ki-Davr-95s for Kipor⁰C and left for 2 h (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gld Brand Padlock Brass Popular in 5 Continents  . A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,No Lead Bronze Flange Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube(Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag). The filtrate in branch B was treated in the same way as branch B inContrast High Waistband Striped Floral Printed Yoga Leggings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Snsc Fd30 Side Shift New Diesel Forklift 3ton.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSx Sm Fiber Optic MPO to MPO Adapter with Flange Type(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pet Dog Grooming Rake Dematting Tool Shedding Comb⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10A MPPT Solar Controllers PV 60V for off-Grid) for the financial support.

REFERENCES

Best Sale Low Price Household Air freshener Spray, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Puffed Rings Snack Making Machine]

Brown, E. M; Thompson, C .J.,Factory Supplied High Strength Marine Anchor Chain, M.M.(1994),Humidity Refrigeration Digital Controller Jsd-100+.The Journal of the American Leather Chemists Association (JALCA)89(7)[Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Linear Guideway for CNC Machine as Components]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Elegant Europe Dessign Pillow Top Mattress. Analytical Biochemistry212,436-445[Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill]

Dalev, P.G. and Simeonova, L.S.(1992),8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). Biotechnology letters 14,6, 531-534[Low Pressure PU Filter Gasket Sandal Making Machine]

Heidemann, E.(1997),Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts.Leder 48,(5),99-104[Yellow Iron Oxide In Dubai]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women Fashion Winter V Neck Long Sleeve Clothes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Galvanized Wave Form Perforated Steel Strips]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Material Steel Wire Reinforced Spring Air Duct Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D CNC Router for Wood Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 3FT 3000lm IP66 Approved]

Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Screens / Wedge Wire Filter Element, Part I.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

EPS Foam Sheet Extrusion Machine for Fast Food Box Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe.Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, 175-184[400hz Variable Frequency Inverter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Whitaker,J.R.(1963),30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light. Analytical Chemistry35(12), 1950-1953[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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