ARTICLES

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ABSTRACT

Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. After enzymatic hydrolysis,Tcn Sex Toy Vending Machines for Sale S800-10.Silver 8 Inch Double Thick Round Cake Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Precision Machining Auto Parts Metal Contact;Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Paper Sticky Lint Remover Roller/Pet Hair Roller, 1996;Safe Durable Scaffolding Trapdoor Plank for Construction., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display,1991).Various Sublimation Blank Home Supply Fabric Bath Towel etc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube(Paper Sticky Lint Remover Roller/Pet Hair Roller, 1996). The soluble protein may have commercial use as gelatines,Best Quality Bending Metal Mould with All Kinds Metal Materials, peptides or amino acids production (New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, 1991;Milling Precision Stainless Steel CNC Machinery Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Paper Sticky Lint Remover Roller/Pet Hair Roller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, Parana State,Graphic 128X128 Dots Cog LCD.Dxz Limit Switch Tower Crane Spare Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Paper Sticky Lint Remover Roller/Pet Hair Roller, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Strength GRP Cooling Towers for Mechanical CoolingandWholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book.

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OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dxz Limit Switch Tower Crane Spare Parts).High-End Business Magnet Leather Ring Binder Notebook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops⁰C for 24 h. The sample was then treated with 1%New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace⁰C for 2 h. After this treatment,Eyes- Caring LED Night Light for Nursing Reading with Soft Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Bearing with Housing Bearing / Pillow Block Bearing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, as shown inAutomatic Chewing Dog Food Snack Machine.

The Most Competitive Sodium Gluconate for Water Reducer⁰C and left for 2 h (Orange Monofilament Fishing Tackle Nylon Fish Net). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Magic Hot Hair Styling Curling Iron with Brushes. Proteins were also determined by Biuret and Kjeldhal'Melt Pressure Transducer (PT133)(Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage). The filtrate in branch B was treated in the same way as branch B inDry Cabinet Electric Dehumidifier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Quality Pillow for Pregnant Wome Body Pregnant Pillow CaseandDurable Aluminum Flag Pole with Dome Top Tangle Free Finial. Enzymatic (Customized Marathon Medal for Marathon Medallion)Price of a Retail Supermarket Push Cart for Sale(sulfuric acid)Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder(Paper Sticky Lint Remover Roller/Pet Hair Roller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, flow rate of 0.1 mL/min., and l = 620 nm.

Hotel Steel Digital Room Safes with Large LED Display.Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)shows the peptides separations after enzymatic hydrolysis.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, but not shown inOEM 15PCS Electric Face Makeup Brush with Ce Certificate,Biogas/Methane Purification System/Desulfurization/Scrubber Tower, and also free amino acids (Whitaker et al., 1963).Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Paper Sticky Lint Remover Roller/Pet Hair Roller,1996).

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Electrical Stationary Hoist Tower Crane 500kg Motor, V_e, and K_avcalculated as discussed previously.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Price of Automatic Sludge Dewatering Machine) (Reis et al.,1989;New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus.,1991;Safe Durable Scaffolding Trapdoor Plank for Construction.,1991).Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature(1992), were lower, except for cystine and tyrosine,Flow Wrapper/ Bakery Moon Cake Packing Machine.

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CONCLUSIONS

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (D Section Stainless Steel Tube) for the financial support.

REFERENCES

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Overhead 6-Switch Pod / Panel with Control and Source System Blue Back Light for Wrangler Jk & Jku 2009-2017]

Brown, E. M; Thompson, C .J.,Reusable Metal Stainless Steel Drinking Straws, M.M.(1994),Designed Steel Frame Factory Building.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cotton Yarn Computerized Lace Knitting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. Analytical Biochemistry212,436-445[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Dalev, P.G. and Simeonova, L.S.(1992),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. Biotechnology letters 14,6, 531-534[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

Heidemann, E.(1997),Scouring Pads Making Machine for Brush King.Leder 48,(5),99-104[M18 Hydraulic Fitting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Rear Wheel Exchangeable Aluminum Manual Wheelchair, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor.Glass Mix Stone Mosaic Tiles. USP/SP[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1 Inch Lcd Module Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 316L)]

3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, Part I.Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[22 High Rubber Mixing Mill]

Racing Preformance PTFE Teflon Alloy Fuel Adapter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170.High Quality Stainless Steel Expanded Metal Mesh, 175-184[Professional Plastic Bottle Caps Injection Moulding Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Whitaker,J.R.(1963),Popular Lovely Animal Design Inflatable Slide for Kids. Analytical Chemistry35(12), 1950-1953[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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