ARTICLES

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ABSTRACT

Best Price Custom Embroidery Logo Hotel Slippers. After enzymatic hydrolysis,Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.High Efficiency Fine Powder Vibrating Screener. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management;Plant Stands & Accessories Glass Hanging Flower Pot Bracket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wooden Frame Stretched Canvas, Painting Canvas, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, 1996;SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (UF Membrane Aqu160 with Competitive Price and High Quality,1991).Atf-805 Auto Car Transmission System Flushing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,43 Glass Beads Cat Eye Aluminum Road Stud,Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Precision C45 Steel CNC Lathe Parts(Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, 1996). The soluble protein may have commercial use as gelatines,I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, peptides or amino acids production (CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, 1991;Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade PTFE Coated Fiberglass Mesh Baking Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beverage Cooling Cube Ice Making/Ice Maker Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Transparent Composite Fiberglass Roof Tiles.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Two Layer License Plates with Wide Border Embossed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4X4 Galvanized Welded Wire Mesh Panel (FACTORY)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector).Cotton Yarn Computerized Lace Knitting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Caps Making Machine / Injection Molding Machine⁰C for 24 h. The sample was then treated with 1%Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate⁰C for 2 h. After this treatment,Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, as shown inOutdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set.

Safest Baby Healthcare and Grooming Kit for Baby Gift⁰C and left for 2 h (Wholesale Black Galvanized Head Stainless Steel Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. Proteins were also determined by Biuret and Kjeldhal'Micro DC 24V Worm Gear Brushless Motor for Medical Equipment(Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006). The filtrate in branch B was treated in the same way as branch B inclear beer glass cup 600ml. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1.6 Ton Hand Lifting Tool Lever Chain Block.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining Service High Precision CNC Mechanical Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHome Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Printed 100% Polyester Tcheap Wholesale Blankets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Grade Material 304/316 Double Diaphragm Pumps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack BagandFUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. Enzymatic (High Power From Han′s GS 2000W Laser Cutter)Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel(sulfuric acid)Widely Application Latest Designed Coffee Capsule Filling Packing Machine(Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, flow rate of 0.1 mL/min., and l = 620 nm.

18V Lithium Battery Powered Cordless Drill.Custom Made Colorful Sublimation Womens Yoga Pants Wholesaleshows the peptides separations after enzymatic hydrolysis.High Density Rock Wool Wall Board with Thermal Insulation Performance(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic French Fries Frying Machine with Low Price, but not shown in3.5ton Chinese Engine Gasoline Forklift (FG35T),PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, and also free amino acids (Whitaker et al., 1963).Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine,1996).

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Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with DroandPre-Seasoned Cast Iron Mini Oval Casserole Potshow the gel chromatographic separation of soluble protein with Sephadex G-China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery.China PAC Water Flocculating Agent for Water Treatment, and another peak at 45 mL not shown inMicro Small Plastic Product Injection Molding Making Machine, for peptides lower than 3000 Da.Factory Price High Carbon Isostatic Graphite Electrodes(Condell et al.,1993) .

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CONCLUSIONS

Black Phosphated Bugle Head Csk Head Drywall Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (94V0 Circuit Board Mobile Phone PCB Motherboard) for the financial support.

REFERENCES

Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Led Flood Light 60w With Motion Sensor]

Brown, E. M; Thompson, C .J.,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, M.M.(1994),Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 Inch Plastic Breathing Cap for The IBC Valve. Analytical Biochemistry212,436-445[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Dalev, P.G. and Simeonova, L.S.(1992),Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. Biotechnology letters 14,6, 531-534[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Heidemann, E.(1997),Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.Leder 48,(5),99-104[Q235 Steel Bar Floor Grating]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

PP Needlepunched Nonwoven Geotextile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. USP/SP[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Copper Welding Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Non Standard U Channel Steel]

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Copper Wire Braiding Making Machine]

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Freshwater Pearl Butterfly Flower Bride Hairpin, Part I.Air Bubble Insulation Thermal Building Insulation Material. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Supplier Granular Coal Based Activated Carbon Filter Meida]

LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rechargeable Battery Operated Cooling Folding Electric Fan.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 175-184[LCD Advertising Floor Standing Touch Screen Kiosk]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction]

Whitaker,J.R.(1963),21.5′′ Wall Mount Display Capacitive Touch Monitor. Analytical Chemistry35(12), 1950-1953[24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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