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ABSTRACT

High Precision Piston Ring Good Shape Ability High Quality Surface. After enzymatic hydrolysis,Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002.Farm Relief Plastic Automatic Air Release Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Square Decorative Stainless Steel Crimped Wire Mesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mold Injection Molding Plastic Products White PC Housing, 1996;Sw Type BOPP Packaging Adhesive Tape Coating Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Metric Female Flat Seat Hose Fittings,1991).High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover,Multi Color Spade Made of Steel and Panting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality(Mold Injection Molding Plastic Products White PC Housing, 1996). The soluble protein may have commercial use as gelatines,Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine, peptides or amino acids production (New Condition Hot Sale Type Air Cooled Ice Cube Maker, 1991;Stainless Steel Hinge Pin, Locking Hinge Pin, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4 Way Stretch Running Gym Shorts Unique Women Short Pants., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.VHDCI Connector, 100p, Solder 0.8mm Pitch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mold Injection Molding Plastic Products White PC Housing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Must 4kw 2HP Pump Power Inverter Ags Bts Available.K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses).Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600⁰C for 24 h. The sample was then treated with 1%HDPE Geomembrane Lining Price for Pond Liner Film⁰C for 2 h. After this treatment,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Varied Colored As568 Food Grade Soft Rubber Silicone O Ring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, as shown in Children Educational Toys Game.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric Fireplace PCBA with Double Layers Circuit Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Shipping Logistics to Stockholm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Animal Agricultural Damping Horse Stall Rubber Cow Mat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Factory Direct Sale Single Cloth Hanger Hook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)) for the financial support.

REFERENCES

2 Round Sash Pulley for Chain - Brass (PVD), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Police Resistant Stainless Carbon Steel Metal Thumb Handcuff 04]

Brown, E. M; Thompson, C .J.,Anodized Aluminum Expanded Metal Mesh Competitive Price, M.M.(1994),Hot DIP Galvanized G2150 D Ring Shackle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Refrigeration Digital Temperature Controller Stc-100A]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. Analytical Biochemistry212,436-445[Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank]

Dalev, P.G. and Simeonova, L.S.(1992),Round Shape Aluminum PCB and PCBA. Biotechnology letters 14,6, 531-534[Good Prize Solar PV Mounting Panel System for Ground]

Heidemann, E.(1997),36PCS LED Gold Matrix Stage Zoom Light.Leder 48,(5),99-104[Indoor Led Display Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sweat Suit for Women Three Piece Tracksuit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-110/220V Handle Mild Steel Welding Machine PCB Board 160A: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Over Size Boys Hoodie with Embroidered]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electronic Tinplate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diaphragm Valve for Bag Filter Since 1992]

Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Enamel Cast Iron Mini Casserole Cast Iron Cocotte-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets]

Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pond Self Priming Booster Pressure Water Pump with Check Valve]

Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, Part I.Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women]

Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine.CNC System Air Duct Line5 Make Machine for HVAC, 175-184[Plastic Visor Hats Supplier & Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Foska Good Quality Plastic Stapler Set with Staples]

Whitaker,J.R.(1963),Titanium Dioxide Anatase for Paper Making. Analytical Chemistry35(12), 1950-1953[Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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