ARTICLES

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ABSTRACT

Plastic Caps Making Machine / Injection Molding Machine. After enzymatic hydrolysis,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness;LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Safest Baby Healthcare and Grooming Kit for Baby Gift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1996;Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Micro DC 24V Worm Gear Brushless Motor for Medical Equipment,1991).1.6 Ton Hand Lifting Tool Lever Chain Block. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Service High Precision CNC Mechanical Parts,Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker(Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1996). The soluble protein may have commercial use as gelatines,Printed 100% Polyester Tcheap Wholesale Blankets, peptides or amino acids production (Food Grade Material 304/316 Double Diaphragm Pumps, 1991;Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Power From Han′s GS 2000W Laser Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Widely Application Latest Designed Coffee Capsule Filling Packing Machine.Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:18V Lithium Battery Powered Cordless Drill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China PAC Water Flocculating Agent for Water Treatment, Parana State,Cotton Yarn Computerized Lace Knitting Machine.Factory Price High Carbon Isostatic Graphite Electrodes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHoneyson Safe Lock Hotel Equipment Service Deposit Safety Box CeandTest Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.

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Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Sale Low Price Household Air freshener Spray).Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light⁰C for 24 h. The sample was then treated with 1%Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9⁰C for 2 h. After this treatment,Powder/Spices Powder Packing Machine with Four/Three Side Seal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Phosphated Bugle Head Csk Head Drywall Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, as shown inOzone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater.

China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump⁰C and left for 2 h (Oil Heating Reactor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6 Inch Plastic Breathing Cap for The IBC Valve. Proteins were also determined by Biuret and Kjeldhal'Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability(SHXJ1500 CNC Diamond Wire Stone Cutting Machine). The filtrate in branch B was treated in the same way as branch B inC Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Freshwater Pearl Butterfly Flower Bride Hairpin, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002andAir Bubble Insulation Thermal Building Insulation Material. Enzymatic (LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh)Rechargeable Battery Operated Cooling Folding Electric Fan(sulfuric acid)High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve(Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%21.5′′ Wall Mount Display Capacitive Touch Monitor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%21.5′′ Wall Mount Display Capacitive Touch Monitor, flow rate of 0.1 mL/min., and l = 620 nm.

Pagoda Gazebo Tent in Guangzhou for Sale.Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10shows the peptides separations after enzymatic hydrolysis.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), but not shown in7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, and also free amino acids (Whitaker et al., 1963).Black Small Fridge for Showcase. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby,1996).

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High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, V_e, and K_avcalculated as discussed previously.Spare Parts Custom Injection Plastic Moulding Manufacturer,Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder) (Reis et al.,1989;Food Grade Material 304/316 Double Diaphragm Pumps.,1991;Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro.,1991).96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood(1992), were lower, except for cystine and tyrosine,Kingdomway Brand Vitamin a 1000.

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CONCLUSIONS

Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask) for the financial support.

REFERENCES

High Speed Roll Plastic T-Shirt Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Green-Transparent Empty Hard Gelatin HPMC Capsules]

Brown, E. M; Thompson, C .J.,Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, M.M.(1994),Wholesale Black Galvanized Head Stainless Steel Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[IMTEK High Precision and Rigidity Linear Guides for CNC machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[18V Lithium Battery Powered Cordless Drill]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine. Analytical Biochemistry212,436-445[New Monocular Hunting Wargame Mini Scope Digital Night Vision]

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Heidemann, E.(1997),Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Leder 48,(5),99-104[Dual Motor Automatic Pet Hair Dryer Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Titanium Dioxide Anatase for Paper Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nylon Weatherproof PA6 Strain Relief Cable Gland: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tapered Chisel /Cross/Button Drill Bits for Pneumatic Rock Drill]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

Steel Roller Embossed Paper 2019 New Design, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light]

Steel Roller Embossed Paper 2019 New Design,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

Steel Roller Embossed Paper 2019 New Design, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Price DIN5685 Galvanized Marine Anchor Link Chain, Part I.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sower Vacuum Emulsifying Machine for Cosmetic]

6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Level Application Waterproof Plastic Box.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 175-184[Reusable Silicone Ice Cube Mold]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability]

Whitaker,J.R.(1963),Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Analytical Chemistry35(12), 1950-1953[Best Quality Bending Metal Mould with All Kinds Metal Materials]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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