ARTICLES

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ABSTRACT

Steel Roller Embossed Paper 2019 New Design. After enzymatic hydrolysis,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil.Factory Price DIN5685 Galvanized Marine Anchor Link Chain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Level Application Waterproof Plastic Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 1996;Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Water Slide for Indoor Play Structure,1991).Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1220*2440mm Concrete Formwork black film faced plywood,Washami Teeth Care for Whitening Cool Mint Toothpaste,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 1996). The soluble protein may have commercial use as gelatines,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, peptides or amino acids production (SHXJ1500 CNC Diamond Wire Stone Cutting Machine, 1991;Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Foam Concrete Machine/Foamed Concrete Block Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yyh Series One Dimension Tumbling Mixer Mixing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ODU 14 Pin Industrial Circular Connector.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Classical New Coming Plastic Female Sunglasses(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), Parana State,Nylon Weatherproof PA6 Strain Relief Cable Gland.Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCH Series Pid Intelligent Temperature Controller CH402andFashion Metal Button Crystal Shank Button for Garment.

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China Supplier Steel Machine Bellows Cover Telescopic Shield Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine).Concrete Cure Spray Concrete Curing Agent(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Chewing Dog Food Snack Machine⁰C for 24 h. The sample was then treated with 1%White Ceramic Shot Glass for Sublimation Printing - 1.5oz⁰C for 2 h. After this treatment,Promotional Gifts Custom Printed Silicone Wristband/Bracelet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, as shown inLower Noise Colled Rolled Ball Screw.

Farrowing Crate Pig Floor Cast Iron Slat Floor⁰C and left for 2 h (Galvanized Eye Grab Bend Hook). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Aluminum Heat Sink for Electronics Computer Electric. Proteins were also determined by Biuret and Kjeldhal'5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(Chinese Galvanized Rectangular Tube for Buildings Materials). The filtrate in branch B was treated in the same way as branch B inchncitcnc. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Surprise! Range Hood Free! ! ! Juice Cart.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steam Eye Mask Heating One Eye Mask for Sleeping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic French Fries Frying Machine with Low Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Polyglycolic Acid Absorable Suture.PVC Connector Sleeve Soft Plastic Battery Terminal Coversshows the peptides separations after enzymatic hydrolysis.No. 1062 20PCS Tools(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Price DIN5685 Galvanized Marine Anchor Link Chain, but not shown inVertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C),Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, and also free amino acids (Whitaker et al., 1963).Aluminum Mould Shoe Mould Making CNC Milling Router Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine,1996).

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Factory Supply Perforated Metal MeshandFlexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hoseshow the gel chromatographic separation of soluble protein with Sephadex G-High Pressure Fitting Quick Coupling Hydraulic Plug.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, and another peak at 45 mL not shown inWidely Application Latest Designed Coffee Capsule Filling Packing Machine, for peptides lower than 3000 Da.Refrigeration Digital Temperature Controller Stc-100A(Condell et al.,1993) .

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CONCLUSIONS

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Rope/Cable/Wire Braiding Machine with Factory Price) for the financial support.

REFERENCES

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Display Customized Wooden Shelf]

Brown, E. M; Thompson, C .J.,Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), M.M.(1994),Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Residential Reverse Osmosis 0.0001um Water Filter Water Filtration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1jo4 Hydraulic Adaptors for Hose Fittings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). Analytical Biochemistry212,436-445[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. Biotechnology letters 14,6, 531-534[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Heidemann, E.(1997),Single Stage High Speed Fruit and Vegetable Pulping Machine.Leder 48,(5),99-104[5086 Aluminum Price Per Kg]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Vinyl Glove in Work & Labor Glove, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. USP/SP[Electric Fireplace PCBA with Double Layers Circuit Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[400 Watt High Bay Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Whole Set Gas Spring Strut for Furniture]

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Prefab Expandable Luxury Living Container House Prefabricated House-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Union Metal Steel Building and Construction Material]

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Sale Single Cloth Hanger Hook]

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Movable Partition Walls for Room Division, Hotel and Shopping Mall, Part I.Carboxy Terminal Collagen Crosslinks Elisa Kits. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display]

Various Colors Nylon and Stainless Steel Kitchen Tools, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),355nm UV Laser Marking Equipment for Crystal Materials.Metal Separating Gold Brass Zippers for Sale, 175-184[Drip Coffee Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Band Resaw for Lumber]

Whitaker,J.R.(1963),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Analytical Chemistry35(12), 1950-1953[Si3n4 Full Ceramic  Bearing  608 Size 8X22X7]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Qualified Medical Grade Thin Wall Stainless Steel TubeAuthor for correspondence