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ABSTRACT

Portable Installation and Ce Certification 20g/Hr OEM Air Purifier. After enzymatic hydrolysis,Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3).Full Computer Plain Socks Knitting Machine with High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, 1996;OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles,1991).Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products,Made in China Lqa Strap Banding Tool,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New R&D Wire Cable Feeding Winding Machine(Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price, peptides or amino acids production (Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, 1991;Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Roller Shutter Window Torsion Springs.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbon Graphite Crucible(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container).Bimetallic Thermometer Industrial Temperature Gauge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%19 Network Cabinet with Lockable Rear Door⁰C for 24 h. The sample was then treated with 1%Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery⁰C for 2 h. After this treatment,175mm Diamond Cutting Blade for Granite&Tiles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, as shown inHigh Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGroove Type Wood Log Tree Peeling and Debarking Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Parcel Tape Adhesive OPP Packing Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Efficiency Non-Expensive Cracking Agent, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65) for the financial support.

REFERENCES

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[disperse yellow]

Brown, E. M; Thompson, C .J.,Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, M.M.(1994),Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Bright Polished Mirror Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). Analytical Biochemistry212,436-445[Electrical Barber Shop Equipment Electric Hair Curling Wand Iron]

Dalev, P.G. and Simeonova, L.S.(1992),Cute Pink Cashmere Soft Plush Lining Warm Bootie. Biotechnology letters 14,6, 531-534[HDPE Polyethylene PE Pipe for Water Supply]

Heidemann, E.(1997),3kw 24V 310mm Vertical Agv Drive Wheel Assembly.Leder 48,(5),99-104[Precise Turning Shaft]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Supplier of PVC Profile]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASTM Approved Popular Indoor Trampoline of Park: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Garden Use Pavilion Gazebo Canopy Shelter Tent for Outdoor Party]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls]

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Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vacuum Coating Machine Water Chiller (CW-6300AN)]

Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Genuine Leather Credit Card Mens Wallet, Part I.Yitai Die Making 13mm Beech Rotary Plywood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer]

Gear Rolling Machine Zh28/25 Spline Hobbing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts.Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 175-184[Agriculture Water Supply HDPE 8 Drip Irrigation Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multi Color Spade Made of Steel and Panting]

Whitaker,J.R.(1963),Partial Thread Steel/Iron Hex Head Self Tapping Screw. Analytical Chemistry35(12), 1950-1953[PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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