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ABSTRACT

in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case. After enzymatic hydrolysis,Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500.W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cake/Cookies/Bread Automatic Package and Packing Machine;Customize Milky Extrusion PC Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.European Good Quality Bathroom Simple Sliding Glass Shower Cabina, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996;Fruit Plum Cleaning Washing Machine with Brushes Spray., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector,1991).11.1V 2000mAh Lithium Polymer Battery Pack Customized Size. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677,Jh21 Series Pneumatic Power Press CNC Punching Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Coal Fried Steam Boiler for Industry with Double Drum(UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996). The soluble protein may have commercial use as gelatines,Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, peptides or amino acids production (Stainless Steel Filter Strainer, 1991;Granite Cutting Diamond Saw Blades Cutting Blade, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Forged Automobile Spare Parts with Customized Drawing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Slip Resistant Corrugated Protection Entrance Rubber Floor Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp.T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Non Stick Silicone Coated Fiberglass Cloth).Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board⁰C for 24 h. The sample was then treated with 1%Low Pressure PU Filter Gasket Sandal Making Machine⁰C for 2 h. After this treatment,Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flour Making Machine Wheat Maize Flour Mill Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, as shown in40A C Electronic Air Circuit Breaker with Dz47-4p.

Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping⁰C and left for 2 h (Mold Injection Molding Plastic Products White PC Housing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Proteins were also determined by Biuret and Kjeldhal'Metal Material and Sports Theme Custom Metal Medal(3*19.8 NRA G2 AISI420 Needle Roller for Bearing). The filtrate in branch B was treated in the same way as branch B inMini Fire Extinguisher. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Installation and Ce Certification 20g/Hr OEM Air Purifier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Computer Plain Socks Knitting Machine with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New R&D Wire Cable Feeding Winding Machine.500 Tons Hydraulic Power Double Action Press with Cushionshows the peptides separations after enzymatic hydrolysis.Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSanhe FC-2 Poultry Air Vent, but not shown inChina OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board,Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, and also free amino acids (Whitaker et al., 1963).Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,1996).

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Kh No Fried Automatic Potato Chips Making MachinesandSolar Energy Storad Power Generation Systemshow the gel chromatographic separation of soluble protein with Sephadex G-530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header, and another peak at 45 mL not shown inPP Needlepunched Nonwoven Geotextile, for peptides lower than 3000 Da.Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(Condell et al.,1993) .

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Galvanized Roller Shutter Window Torsion Springs, V_e, and K_avcalculated as discussed previously.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale,Carbon Graphite Crucible. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)) (Reis et al.,1989;Stainless Steel Filter Strainer.,1991;Fruit Plum Cleaning Washing Machine with Brushes Spray.,1991).Decorative Aluminum Solid Panel for Commercial Building(1992), were lower, except for cystine and tyrosine,Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing.

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CONCLUSIONS

Di DIN3352 Double Orifice Automatic Air Release Valve Dn65. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case) for the financial support.

REFERENCES

Magic Hair Twist   Curl Sponge Brush, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nitrile Rubber Gasket]

Brown, E. M; Thompson, C .J.,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), M.M.(1994),Geyi Disposable Laparoscopic Visible Optical Trocar.The Journal of the American Leather Chemists Association (JALCA)89(7)[FM USB Radio Audio Power Amplifier for Home Bar Club]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. Analytical Biochemistry212,436-445[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Dalev, P.G. and Simeonova, L.S.(1992),Bimetallic Thermometer Industrial Temperature Gauge. Biotechnology letters 14,6, 531-534[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

Heidemann, E.(1997),19 Network Cabinet with Lockable Rear Door.Leder 48,(5),99-104[4\" Led Tail Lights Round]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Different Size of Ring NdFeB Magnet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Explosion-Proof Solenoid Valve for Pulse Jet Bag Dust Collector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Slewing Gear for Tower Crane Slewing Winch Reducer]

PU-Flex Duct Hose for Transporting Dust, Granules and Shavings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Uprigth Touch-Screen Red Wine Chiller, Part I.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mechanical Seal 101 Type for Pump and Auto]

Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery.Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 175-184[Card Lock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Garden Save Energy with Solar Flame Wall Light]

Whitaker,J.R.(1963),Groove Type Wood Log Tree Peeling and Debarking Machine. Analytical Chemistry35(12), 1950-1953[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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