ARTICLES

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ABSTRACT

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. After enzymatic hydrolysis,Explosion-Proof Housing Sealing Dispensing Machine.Tube Mills for Thin Wall Tubing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core;Car Badge Custom Emblem Auto Logo Sign for Advertising; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), 1996;Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rear Wheel Exchangeable Aluminum Manual Wheelchair,1991).90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Graphic 128X128 Dots Cog LCD,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tcn Sex Toy Vending Machines for Sale S800-10(Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), 1996). The soluble protein may have commercial use as gelatines,Silver 8 Inch Double Thick Round Cake Board, peptides or amino acids production (High Precision Machining Auto Parts Metal Contact, 1991;Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Paper Sticky Lint Remover Roller/Pet Hair Roller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Safe Durable Scaffolding Trapdoor Plank for Construction. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.Various Sublimation Blank Home Supply Fabric Bath Towel etc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Good Price Stand-up Zipper Doy Pouch Filling Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flat Pack Temperary Container House 20FT 40FT).Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10⁰C for 24 h. The sample was then treated with 1%360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)⁰C for 2 h. After this treatment,Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Machine Cube Ice Machine for Commercial Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Christmas Decoration Rose Flower LED Light String, as shown inHigh Quality Engieered Veneer Faced Commericial Plywood.

D Section Stainless Steel Tube⁰C and left for 2 h (7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mint Flavor 50ml Home Foam Toothpaste. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. Proteins were also determined by Biuret and Kjeldhal'Dxz Limit Switch Tower Crane Spare Parts(OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock). The filtrate in branch B was treated in the same way as branch B inEngine Crankshaft_Engine Crank Shaft. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High-End Business Magnet Leather Ring Binder Notebook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eyes- Caring LED Night Light for Nursing Reading with Soft Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Bearing with Housing Bearing / Pillow Block Bearing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Quality Bending Metal Mould with All Kinds Metal Materials) for the financial support.

REFERENCES

Biogas/Methane Purification System/Desulfurization/Scrubber Tower, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Green Underwater Led Lights]

Brown, E. M; Thompson, C .J.,Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, M.M.(1994),Popular Custom School Uniform Woven Badge Patch with Merrow Border.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor. Analytical Biochemistry212,436-445[Digital Programmable Ntc Temperature Controller]

Dalev, P.G. and Simeonova, L.S.(1992),Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. Biotechnology letters 14,6, 531-534[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

Heidemann, E.(1997),Electrical Stationary Hoist Tower Crane 500kg Motor.Leder 48,(5),99-104[Auto Parts Car Mat for Nissan Livina Geniss]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory OEM CNC Machined Gear Motor Shaft]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4 in 1 Multifunctional Beauty Machine: Cryolipolysis Coolsculpting Vacuum Cavitation RF Laser for Whole Body Fat Removal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ COB Stn LCD Display with 8080 Interface]

Flow Wrapper/ Bakery Moon Cake Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag]

Flow Wrapper/ Bakery Moon Cake Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carboxy Terminal Collagen Crosslinks Elisa Kits]

Flow Wrapper/ Bakery Moon Cake Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, Part I.Reusable Metal Stainless Steel Drinking Straws. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Popular Custom School Uniform Woven Badge Patch with Merrow Border]

Designed Steel Frame Factory Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, 175-184[(Midtech Industry) Plastic HDPE/PE Ocean Marine Pedal Profile Board Machine Extruder Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pump Flower Sprinkler with Automatic Watering System]

Whitaker,J.R.(1963),Scouring Pads Making Machine for Brush King. Analytical Chemistry35(12), 1950-1953[Rear Wheel Exchangeable Aluminum Manual Wheelchair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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