ARTICLES

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ABSTRACT

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. After enzymatic hydrolysis,Braiding Machine for Weaving Flexible Metal Hose.Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, 1996;ABC-508 Brake System Cleaning and Fluid Exchanging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polypropylene Woven Bag for Sludge Dewatering,1991).China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing,High Quality Industrial Grade Anhydrous Sodium Sulfate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Factory Supply Design Cat Tree Pet Product(Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, 1996). The soluble protein may have commercial use as gelatines,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), peptides or amino acids production (Peb Steel Structure Building Materials H-Section I Beam Steel, 1991;Aluminum Oxide Non Woven Polishing Wheel for Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CAT6A FTP Patch Panel (WD6A-012)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Famous Brand Luxury Packaging Box Gift Box.High Qualified Medical Grade Thin Wall Stainless Steel Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Rope/Cable/Wire Braiding Machine with Factory Price).Picc Puncture Care Kit(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth⁰C for 24 h. The sample was then treated with 1%AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C for 2 h. After this treatment,34*25cm Office File Pocket Felt A4 Document Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP Needlepunched Nonwoven Geotextile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Temperaturethermocouple Male and Female Plug, as shown inCustomized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women.

34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts⁰C and left for 2 h (Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Price Custom Embroidery Logo Hotel Slippers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Proteins were also determined by Biuret and Kjeldhal'High Efficiency Fine Powder Vibrating Screener(Long Distance Measurement Power Laser Digital Meter). The filtrate in branch B was treated in the same way as branch B in7.5*3.55 Fuel Injector Viton FKM NBR Acm Rubber O Ring Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plant Stands & Accessories Glass Hanging Flower Pot Bracket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWooden Frame Stretched Canvas, Painting Canvas(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/UF Membrane Aqu160 with Competitive Price and High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Atf-805 Auto Car Transmission System Flushing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Jar Blowerand43 Glass Beads Cat Eye Aluminum Road Stud. Enzymatic (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041))High Precision C45 Steel CNC Lathe Parts(sulfuric acid)I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max(Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Adhesive Powder for Skim Coat/Wall Putty HPMC. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Cotton Yarn Computerized Lace Knitting Machine, M.M.(1994),Plastic Caps Making Machine / Injection Molding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. Analytical Biochemistry212,436-445[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Biotechnology letters 14,6, 531-534[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Heidemann, E.(1997),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.Leder 48,(5),99-104[Pink Ribbon Breast Cancer Awareness Lapel Pin]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Leak Proof]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.outdoorgamefactory.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Micro DC 24V Worm Gear Brushless Motor for Medical Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.6 Ton Hand Lifting Tool Lever Chain Block, Part I.CNC Machining Service High Precision CNC Mechanical Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.Printed 100% Polyester Tcheap Wholesale Blankets, 175-184[Enamel Cookware Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Whitaker,J.R.(1963),Food Grade Material 304/316 Double Diaphragm Pumps. Analytical Chemistry35(12), 1950-1953[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)Author for correspondence