ARTICLES

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ABSTRACT

3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. After enzymatic hydrolysis,High Strength New 0.18mm Molybdenum Wire.Structural Synthetic Fiber for Concrete. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer;Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tct Circular Saw Blade for Grass Cutting, 1996;Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Picc Puncture Care Kit,1991).Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture,34*25cm Office File Pocket Felt A4 Document Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PP Needlepunched Nonwoven Geotextile(Tct Circular Saw Blade for Grass Cutting, 1996). The soluble protein may have commercial use as gelatines,High Temperaturethermocouple Male and Female Plug, peptides or amino acids production (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, 1991;Best Price Custom Embroidery Logo Hotel Slippers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Fine Powder Vibrating Screener.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tct Circular Saw Blade for Grass Cutting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plant Stands & Accessories Glass Hanging Flower Pot Bracket.Wooden Frame Stretched Canvas, Painting Canvas-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), Parana State,High Qualified Medical Grade Thin Wall Stainless Steel Tube.High Precision C45 Steel CNC Lathe Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tct Circular Saw Blade for Grass Cutting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHydrophobic Excellent Thermal Insulation Silica Aerogel PowderandNew Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.

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I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan).CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1⁰C for 24 h. The sample was then treated with 1%Food Grade PTFE Coated Fiberglass Mesh Baking Sheet⁰C for 2 h. After this treatment,Beverage Cooling Cube Ice Making/Ice Maker Machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Transparent Composite Fiberglass Roof Tiles, as shown inDifferent Size of Ring NdFeB Magnet.

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125⁰C and left for 2 h (High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Two Layer License Plates with Wide Border Embossed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. Proteins were also determined by Biuret and Kjeldhal'Really Microfiber PVC Leather for Sofa Furniture(Slewing Gear for Tower Crane Slewing Winch Reducer). The filtrate in branch B was treated in the same way as branch B inElectrophoresis Power Supply. The final soluble protein shown were used for peptides separations using gel permeation chromatography,94V0 Circuit Board Mobile Phone PCB Motherboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4X4 Galvanized Welded Wire Mesh Panel (FACTORY)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cotton Yarn Computerized Lace Knitting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Caps Making Machine / Injection Molding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Atf-805 Auto Car Transmission System Flushing Machine) for the financial support.

REFERENCES

Widely Application Latest Designed Coffee Capsule Filling Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.ahnoyark.com]

Brown, E. M; Thompson, C .J.,Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, M.M.(1994),18V Lithium Battery Powered Cordless Drill.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Density Rock Wool Wall Board with Thermal Insulation Performance. Analytical Biochemistry212,436-445[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. Biotechnology letters 14,6, 531-534[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

Heidemann, E.(1997),Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask.Leder 48,(5),99-104[Field Quick Assembly LC Upc Single Mode Fiber Optic LC/Upc Fast Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away]

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery.China PAC Water Flocculating Agent for Water Treatment. USP/SP[14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price High Carbon Isostatic Graphite Electrodes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Organic Fruit And Veg Box Delivery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Machining Non-Standard Hardware Parts]

Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light, Part I.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Southeast Asia Healthy Soft Integrated Latex Foam Mattress]

Whitaker,J.R.(1963),China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. Analytical Chemistry35(12), 1950-1953[Classical New Coming Plastic Female Sunglasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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