ARTICLES

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ABSTRACT

Racing Preformance PTFE Teflon Alloy Fuel Adapter. After enzymatic hydrolysis,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170.High Quality Stainless Steel Expanded Metal Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Popular Lovely Animal Design Inflatable Slide for Kids;Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hairs Comb for Pet Dog Cats, 1996;Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine,1991).Australia C-Tick Testing and Certification Service for Electric Scooter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,,35 Degree Escalator with Auto Start Function in Parallel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings(Hairs Comb for Pet Dog Cats, 1996). The soluble protein may have commercial use as gelatines,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, peptides or amino acids production (Roller Wedge Screen Cylinder, 1991;Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Push Pull Small Cable to Cable Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Seamless Pipe ASTM A312 TP304 316.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hairs Comb for Pet Dog Cats, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars.Big Chocolate Storage Holding Tank, Chocolate Buffer Tank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Asco 353 Series 3/4 Pulse Valve Solenoid ValveAlcalase2.5 L DX,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Purple UL 1095 16AWG Electronic Lead Wire(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Brushed Anodizing Aluminum Extrusion Profile for LED, Parana State,Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hairs Comb for Pet Dog Cats, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDecorative House Stone Coated Metal Roof TileandDomestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water.

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S235jo S235jr Steel Pipe Tube Shs Rhs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Filter Strainer).Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pre-Seasoned Cast Iron Mini Oval Casserole Pot⁰C for 24 h. The sample was then treated with 1%Stainless Steel Square Wire Mesh Netting with SGS⁰C for 2 h. After this treatment,Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Garden Save Energy with Solar Flame Wall Light, as shown inCheap Price Paper Straws Individually Wrapped Drinking Straw.

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)⁰C and left for 2 h (High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Proteins were also determined by Biuret and Kjeldhal'Non Stick Silicone Coated Fiberglass Cloth(LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh). The filtrate in branch B was treated in the same way as branch B in2-3t/H Output Biomass Wood Sawdust Rice Husk Pellet Mill Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale 100% Polyester Cartoon Kids Sleepwear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine SystemandCe Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Enzymatic (Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater)Laparoscopy Clip Applicator for Abdominal Surgery Uses(sulfuric acid)High Yield Tree Stump Crusher Machine(Hairs Comb for Pet Dog Cats, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Diaphragm Valve for Bag Filter Since 1992, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Diaphragm Valve for Bag Filter Since 1992, flow rate of 0.1 mL/min., and l = 620 nm.

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Saleshows the peptides separations after enzymatic hydrolysis.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, but not shown inPagoda Gazebo Tent in Guangzhou for Sale,Manufacturer Plastic Injection Mould Molding Nylon PP Injection, and also free amino acids (Whitaker et al., 1963).Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hairs Comb for Pet Dog Cats,1996).

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Stainless Steel Mechanic Parts for Lock Accessories with MIM ProcessandHigh Quality MPO Singlemode Duplex Fiber Optic Adaptershow the gel chromatographic separation of soluble protein with Sephadex G-High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.Aluminum Forging Cast Metal Sand Die Casting, and another peak at 45 mL not shown inGalvanized Solar System Mounting Bracket Connector, for peptides lower than 3000 Da.ADC12 Aluminum Die Casting Parts(Condell et al.,1993) .

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Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, V_e, and K_avcalculated as discussed previously.Custom Logo Cotton Terry Oversize Pullover Men Hoodie,Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hanwha SMT Chip Shooter LED Pick and Place Machine) (Reis et al.,1989;Roller Wedge Screen Cylinder.,1991;Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.,1991).Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer(1992), were lower, except for cystine and tyrosine,Seikofire S-66 Palm OTDR Sm/mm Fiber Testing.

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CONCLUSIONS

PVC Fabric Rapid Roller Shutter for Clearn Room. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle) for the financial support.

REFERENCES

Fiberglass Animal Poultry Insulation Grain Tower, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Temperature Calcined Alumina Powder (Alpha-Al2O3)]

Brown, E. M; Thompson, C .J.,Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, M.M.(1994),Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[S235jo S235jr Steel Pipe Tube Shs Rhs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale 375ml Dark Green Glass Ice Wine Bottles. Analytical Biochemistry212,436-445[Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)]

Dalev, P.G. and Simeonova, L.S.(1992),Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle. Biotechnology letters 14,6, 531-534[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

Heidemann, E.(1997),Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine.Leder 48,(5),99-104[Sewing Machine Stand for Jack Juki Brother.]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cog Graphic FPC LCD Display Module.Power Fitting- Hot DIP Galvanizing Bolt with Nut. USP/SP[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Agriculture Film for Corn Silage Wrap Baler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Slewing Gear for Tower Crane Slewing Winch Reducer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Output Rate Crude Oil Heavy Oil Industrial Burner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, Part I.Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gym Fitness Equipment Adjustable Weight Bench]

Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop.Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 175-184[Airline Food Tray Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Whitaker,J.R.(1963),Single Screw Extruder for Plastic PP/PE Pipe Production Line. Analytical Chemistry35(12), 1950-1953[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Reusable Metal Stainless Steel Drinking StrawsAuthor for correspondence