ARTICLES

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ABSTRACT

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. After enzymatic hydrolysis,Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card.200W UFO LED High Bay Light Fixture for Interior Exterior Applications. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2);13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bathroom False Plastic Board Interior Decorative 3D Wall Panels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mushroom Head Stud Sert, 1996;Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket,1991).High Borosilicate Glass Baking Dish Set Clear. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen,Embossing Roller for Tissue Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel(Mushroom Head Stud Sert, 1996). The soluble protein may have commercial use as gelatines,Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, peptides or amino acids production (Colorful  Reflective Sticker Reflective Tape, 1991;Concrete Spare Part Electrical System Central Distribution Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.50Hz to 60Hz Frequency Inverter for Water Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mushroom Head Stud Sert, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325).4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cable Tray with ′t′ Slots(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup).10 Gauge Galvanized Welded Wire Mesh for Construction Using(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Factory Inline Hockey Skate Puck⁰C for 24 h. The sample was then treated with 1%0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector⁰C for 2 h. After this treatment,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable PS Foam Fast Food Box Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), as shown inBamboo Tablet Stand Laptop Rack Bt-2212.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500t Plywood Hot Press Woodworking Machine (12-25 layers). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10ml Round Clear Nail Polish Bottle with Brush Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Medical Human Body Thermometer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss Reduced Port 2000wog 1PC Screwed Ball Valve) for the financial support.

REFERENCES

Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Bags Packing Machine for Medical Cotton Roll Between 20 Grams to 1000 Grams. Medical Lap Making Machines Absorbent Cotton Carding Machine for Batt]

Brown, E. M; Thompson, C .J.,Greenhouse Fixing Film Profile Lock Channel and Spring Wire, M.M.(1994),30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products.The Journal of the American Leather Chemists Association (JALCA)89(7)[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. Analytical Biochemistry212,436-445[Pagoda Gazebo Tent in Guangzhou for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. Biotechnology letters 14,6, 531-534[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Solar System Mounting Bracket Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Supplier Granular Coal Based Activated Carbon Filter Meida: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cluster Indian Style Belly Ring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hanwha SMT Chip Shooter LED Pick and Place Machine]

Glass Cutting Table/CNC Glass Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),12 Bottles Portable Home Ues Thermoelectric Wine Chiller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine]

Glass Cutting Table/CNC Glass Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mining Machinery Sand Vibrating Sieve for River Sand]

Glass Cutting Table/CNC Glass Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, Part I.Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[S235jo S235jr Steel Pipe Tube Shs Rhs]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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