ARTICLES

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ABSTRACT

SHXJ1500 CNC Diamond Wire Stone Cutting Machine. After enzymatic hydrolysis,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball;Yyh Series One Dimension Tumbling Mixer Mixing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ODU 14 Pin Industrial Circular Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, 1996;Classical New Coming Plastic Female Sunglasses., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Double Sided Rigid PCB Circuit Board Manufacturer,1991).Robot of World Robotics Competition. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Rope/Cable/Wire Braiding Machine with Factory Price,Aluminum PCB Circuit Board SMD PCBA LED PCB,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)(Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, 1996). The soluble protein may have commercial use as gelatines,Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, peptides or amino acids production (China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, 1991;Concrete Cure Spray Concrete Curing Agent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Chewing Dog Food Snack Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White Ceramic Shot Glass for Sublimation Printing - 1.5oz.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Promotional Gifts Custom Printed Silicone Wristband/Bracelet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door.8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Farrowing Crate Pig Floor Cast Iron Slat Floor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce).55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security⁰C for 24 h. The sample was then treated with 1%Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping⁰C for 2 h. After this treatment,Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)T90 High Power Latching Relay Mini Power Relay, as shown inTest Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Mould Shoe Mould Making CNC Milling Router Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure Fitting Quick Coupling Hydraulic Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Refrigeration Digital Temperature Controller Stc-100A⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Various Colors Nylon and Stainless Steel Kitchen Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover) for the financial support.

REFERENCES

355nm UV Laser Marking Equipment for Crystal Materials, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Woman style Factory & Manufacturer]

Brown, E. M; Thompson, C .J.,Metal Separating Gold Brass Zippers for Sale, M.M.(1994),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.The Journal of the American Leather Chemists Association (JALCA)89(7)[from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Speed Cable Wire Winding Braiding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Refrigeration Digital Thermometer. Analytical Biochemistry212,436-445[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Dalev, P.G. and Simeonova, L.S.(1992),BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L). Biotechnology letters 14,6, 531-534[IMTEK High Precision and Rigidity Linear Guides for CNC machinery]

Heidemann, E.(1997),Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making.Leder 48,(5),99-104[Universal Aluminum Foil Induction Bottle Cap Seal Liner]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[18V Lithium Battery Powered Cordless Drill]

Washami Teeth Care for Whitening Cool Mint Toothpaste, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.Two Roll Rubber & Plastic Mixing Mills Tire Machine. USP/SP[New Monocular Hunting Wargame Mini Scope Digital Night Vision]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Forged Steel Pressure Sealed Bonnet Globe Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Various Aluminum Hardware Fastening/Real Accessories]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Power From Han′s GS 2000W Laser Cutter]

Hot Sale 25kw Grid Tied Home Solar Panel System Kit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Car Portable Fridge 10L Mini Fridge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Titanium Dioxide Anatase for Paper Making]

Hot Sale 25kw Grid Tied Home Solar Panel System Kit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Hot Sale 25kw Grid Tied Home Solar Panel System Kit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, Part I.Mini Small Garden Excavator Digging Work. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Offshore Drilling Platform Air Winch for Marine Oilfields.Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, 175-184[Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light]

Whitaker,J.R.(1963),Heavy Industry Electric Transport Vehicle on Rails. Analytical Chemistry35(12), 1950-1953[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sower Vacuum Emulsifying Machine for CosmeticAuthor for correspondence