ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Forging Cast Metal Sand Die Casting

ABSTRACT

ADC12 Aluminum Die Casting Parts. After enzymatic hydrolysis,Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale.Custom Logo Cotton Terry Oversize Pullover Men Hoodie. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Fabric Rapid Roller Shutter for Clearn Room, 1996;Fiberglass Animal Poultry Insulation Grain Tower., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon,1991).Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale 375ml Dark Green Glass Ice Wine Bottles,Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine(PVC Fabric Rapid Roller Shutter for Clearn Room, 1996). The soluble protein may have commercial use as gelatines,Cog Graphic FPC LCD Display Module, peptides or amino acids production (Power Fitting- Hot DIP Galvanizing Bolt with Nut, 1991;Slewing Gear for Tower Crane Slewing Winch Reducer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Fabric Rapid Roller Shutter for Clearn Room, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System.Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 ApprovedAlcalase2.5 L DX,Single Screw Extruder for Plastic PP/PE Pipe Production Line, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Gold Star Square Glass Candle Holder for Wedding Decoration, Parana State,Aluminum Forging Cast Metal Sand Die Casting.11kg 24L Low Pressure LPG Gas Cylinder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVC Fabric Rapid Roller Shutter for Clearn Room, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDie Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser PartsandDia. 5.5mm Bonding ND: YAG Laser Rod Lens.

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Commercial Use Click Lock Standard Spc Vinyl Plank Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door).Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng⁰C for 24 h. The sample was then treated with 1%Precision Customized OEM Auto Hardware Lathe CNC Machining Parts⁰C for 2 h. After this treatment,White Color Enamel Bowls with High Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, as shown inNew Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints.

Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board⁰C and left for 2 h (Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Southeast Asia Healthy Soft Integrated Latex Foam Mattress. Proteins were also determined by Biuret and Kjeldhal'12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad(Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)). The filtrate in branch B was treated in the same way as branch B inAll Type Heads Hydraulic Hose Fittings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Filter System Lamella Clarifier for River Water Treatment System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCabinet Shelf Support Pin Furniture Hardware Fittings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Anesthesia Breathing Circuit Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck.Flat Pack Temperary Container House 20FT 40FTshows the peptides separations after enzymatic hydrolysis.Road Safety Warning Barricade LED Solar Powered Blinking Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Engieered Veneer Faced Commericial Plywood, but not shown in7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting,Manufacturer Customized Plastic Injection Molding, and also free amino acids (Whitaker et al., 1963).Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Fabric Rapid Roller Shutter for Clearn Room,1996).

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Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27andPositive Drive Turn Belt for Spiral Freezing Equipmentshow the gel chromatographic separation of soluble protein with Sephadex G-Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve.Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine, and another peak at 45 mL not shown inYitai Die Making 13mm Beech Rotary Plywood, for peptides lower than 3000 Da.60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light(Condell et al.,1993) .

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Solar Energy Storad Power Generation System, V_e, and K_avcalculated as discussed previously.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K),Custom Aluminum Cover Frame Half Aluminum Cover Profile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle) (Reis et al.,1989;Power Fitting- Hot DIP Galvanizing Bolt with Nut.,1991;Fiberglass Animal Poultry Insulation Grain Tower.,1991).Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(1992), were lower, except for cystine and tyrosine,DC24V 36W Solar Fan Motor PCBA Card.

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CONCLUSIONS

New Monocular Hunting Wargame Mini Scope Digital Night Vision. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck) for the financial support.

REFERENCES

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[P8 SMD Outdoor Full Color LED Display Screen Videowall for Advertising]

Brown, E. M; Thompson, C .J.,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, M.M.(1994),Italy Design Good Quality Hydraulic Motorcycle Lift Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Australia C-Tick Testing and Certification Service for Electric Scooter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. Analytical Biochemistry212,436-445[Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints]

Dalev, P.G. and Simeonova, L.S.(1992),Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids. Biotechnology letters 14,6, 531-534[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Heidemann, E.(1997),New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.Leder 48,(5),99-104[Wood Milling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block]

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Portable Telescopic Straw Metal Drinking Straw with Brush Case.Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. USP/SP[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Etching Rear Trunk Sill Plates for Hyundai Tucson: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Digital Programmable Ntc Temperature Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[E9781 Goodyear 1r12-303/12-603 Rubber Air Spring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lab Equipment Sr Paper Pulp Beating Freeness Test Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag]

Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Menthol E Liquid E Juice for EGO E Cigarettes]

Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, Part I.Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory OEM CNC Machined Gear Motor Shaft]

Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making.Double Roller Rotary Wood Peeling Machine with CE for Sale, 175-184[Ceramic Vacuum Disc Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

Whitaker,J.R.(1963),5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter. Analytical Chemistry35(12), 1950-1953[ COB Stn LCD Display with 8080 Interface]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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