ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Beverage Cooling Cube Ice Making/Ice Maker Machinery. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank

ABSTRACT

Transparent Composite Fiberglass Roof Tiles. After enzymatic hydrolysis,Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.Two Layer License Plates with Wide Border Embossed. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electrical Barber Shop Equipment Electric Hair Curling Wand Iron;Really Microfiber PVC Leather for Sofa Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.94V0 Circuit Board Mobile Phone PCB Motherboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, 1996;PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Adhesive Powder for Skim Coat/Wall Putty HPMC,1991).4X4 Galvanized Welded Wire Mesh Panel (FACTORY). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cotton Yarn Computerized Lace Knitting Machine,Plastic Caps Making Machine / Injection Molding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate(Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, 1996). The soluble protein may have commercial use as gelatines,Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, peptides or amino acids production (Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, 1991;LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Safest Baby Healthcare and Grooming Kit for Baby Gift., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Micro DC 24V Worm Gear Brushless Motor for Medical Equipment.1.6 Ton Hand Lifting Tool Lever Chain Block-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machining Service High Precision CNC Mechanical Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Power From Han′s GS 2000W Laser Cutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging).Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Widely Application Latest Designed Coffee Capsule Filling Packing Machine⁰C for 24 h. The sample was then treated with 1%Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans⁰C for 2 h. After this treatment,18V Lithium Battery Powered Cordless Drill. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Density Rock Wool Wall Board with Thermal Insulation Performance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, as shown inCircular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1.

Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask⁰C and left for 2 h (High Temperaturethermocouple Male and Female Plug). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China PAC Water Flocculating Agent for Water Treatment. Proteins were also determined by Biuret and Kjeldhal'Factory Price High Carbon Isostatic Graphite Electrodes(New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle). The filtrate in branch B was treated in the same way as branch B inWeir Slurry Pumps. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Powder/Spices Powder Packing Machine with Four/Three Side Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Phosphated Bugle Head Csk Head Drywall Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Electrical PV DC Solar CableandChina Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. Enzymatic (Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence)6 Inch Plastic Breathing Cap for The IBC Valve(sulfuric acid)Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability(Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Printed 100% Polyester Tcheap Wholesale Blankets) for the financial support.

REFERENCES

Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Paper Ice Cream Cups]

Brown, E. M; Thompson, C .J.,Black Small Fridge for Showcase, M.M.(1994),Microwave Motion Sensor Module Doppler Radar Detector (HW-M10).The Journal of the American Leather Chemists Association (JALCA)89(7)[Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2ton R Type Electric Wire Rope Hoist with Single Speed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile. Analytical Biochemistry212,436-445[Peb Steel Structure Building Materials H-Section I Beam Steel]

Dalev, P.G. and Simeonova, L.S.(1992),Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. Biotechnology letters 14,6, 531-534[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Spare Parts Custom Injection Plastic Moulding Manufacturer.Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. USP/SP[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Accept OEM New Products Team Various Colors Gold Ribbon]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lower Price Gi/PPGI Roofing Sheet in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Kingdomway Brand Vitamin a 1000, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Kingdomway Brand Vitamin a 1000,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Kingdomway Brand Vitamin a 1000, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed Roll Plastic T-Shirt Bag Making Machine, Part I.Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[S Series China Generator Helical-Worm Crushers Reduction Box]

Wholesale Black Galvanized Head Stainless Steel Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine.45*60cm Anti-Fatigue ESD Rubber Floor Mat, 175-184[High Quality Dzg Tank Rotator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pre-Seasoned Cast Iron Baking Stone Cookware]

Whitaker,J.R.(1963),Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. Analytical Chemistry35(12), 1950-1953[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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