ARTICLES

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ABSTRACT

White Color Enamel Bowls with High Quality. After enzymatic hydrolysis,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board;Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Southeast Asia Healthy Soft Integrated Latex Foam Mattress, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 1996;Water Filter System Lamella Clarifier for River Water Treatment System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C),1991).Cabinet Shelf Support Pin Furniture Hardware Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reasonable Price High Quality Nylon Cable Gland,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Anesthesia Breathing Circuit Tube(12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 1996). The soluble protein may have commercial use as gelatines,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, peptides or amino acids production (Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element, 1991;New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Counter-Clip Fluorine-Lined Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DC Brushless Motor for CNC Machine with ISO9001. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck.Road Safety Warning Barricade LED Solar Powered Blinking Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer Customized Plastic Injection Molding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Aluminum Cover Frame Half Aluminum Cover Profile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall).Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DC24V 36W Solar Fan Motor PCBA Card⁰C for 24 h. The sample was then treated with 1%New Monocular Hunting Wargame Mini Scope Digital Night Vision⁰C for 2 h. After this treatment,Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Italy Design Good Quality Hydraulic Motorcycle Lift Table, as shown inHeat Pump Food Cabinet Dog Food Pet Food Dryer Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Etching Rear Trunk Sill Plates for Hyundai Tucson. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ladle Refining Melting Steel Furnace Casting Ladle.Customized Souvenirs Tin Metal Number & Name Plate for Carshows the peptides separations after enzymatic hydrolysis.Lishui Factory High Quality Metal Rolling Ball Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inXPS Extruded Polystyrene Foam Board, but not shown inCustom Rubber Product Seal O-Ring /NBR70 O Ring,Y-Spring Threaded Check Valve, 800 Wog, and also free amino acids (Whitaker et al., 1963).OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad,1996).

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CONCLUSIONS

Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine) for the financial support.

REFERENCES

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom 5 Axis Cheap CNC Machining Parts CNC Prototypes]

Brown, E. M; Thompson, C .J.,Pure Sine Wave Power Frequency Inverter, M.M.(1994),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Colorful 100% Cotton Hotel Face Towels. Analytical Biochemistry212,436-445[Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S). Biotechnology letters 14,6, 531-534[Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree]

Heidemann, E.(1997),33kv Dry Type Resin Casted Distribution Transformer.Leder 48,(5),99-104[Air Shaft Company]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Roller Embossed Paper 2019 New Design]

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27.Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate. USP/SP[Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[No Lead Bronze Flange Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Size 000 Clear Pullulan Vegetarian Capsules: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1220 Fork Length 3-Wheel Electric Forklift Truck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[E-MARK Certified 5-Inch 2.4G Wireless Security System Backup Camera for Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Miniature Ball Bearing (616)]

Sinicline 2018 Hot Sell Custom Printing Thank You Cards, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Black Red Open Net Spandex Nylon Sexy Lingerie-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell Mechanical Bar Screen for Water Treatment]

Sinicline 2018 Hot Sell Custom Printing Thank You Cards,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500]

Sinicline 2018 Hot Sell Custom Printing Thank You Cards, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, Part I.Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone]

LED Emergency Exit Lights, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, 175-184[Umbrella Heater]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Worm Gear Operation Wafer Lined F3 Butterfly Valve]

Whitaker,J.R.(1963),Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine. Analytical Chemistry35(12), 1950-1953[China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Jar OpenerAuthor for correspondence