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ABSTRACT

Chicken Cage Mesh Hexagonal Wire Mesh Machine. After enzymatic hydrolysis,110/220V Handle Mild Steel Welding Machine PCB Board 160A.Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Enamel Cast Iron Mini Casserole Cast Iron Cocotte;Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, 1996;Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC System Air Duct Line5 Make Machine for HVAC,1991).Titanium Dioxide Anatase for Paper Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,40A C Electronic Air Circuit Breaker with Dz47-4p,Pipe Thermostat with Sensor Ce Certificate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Walking Long Distance Surveying Instrument Meter Measuring Wheel(Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, 1996). The soluble protein may have commercial use as gelatines,Chinese Supplier of PVC Profile, peptides or amino acids production (Tianjin Manufacturer PTFE Coated Butterfly Valve, 1991;Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Prices of 20W 40W 60W Solar Street Lights., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sinicline Custom Brand with Plastic Pouch Hangtag for Hats.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ALS Stage 5W RGB Animation Laser Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Price of Automatic Sludge Dewatering Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized 3D Medal for Religious Medal Gift).Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Worm Gear Operation Wafer Lined F3 Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad⁰C for 2 h. After this treatment,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Positive Drive Turn Belt for Spiral Freezing Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese Herb Medicine Angelica Root Extract, as shown inStainless Steel Square Wire Mesh Netting with SGS.

S Series China Generator Helical-Worm Crushers Reduction Box⁰C and left for 2 h (Charms for Jewelry Making (Sold Per Bag 10PCS Inside)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Testo 174t Mini Temperature Data Logger No. 0572 1560. Proteins were also determined by Biuret and Kjeldhal'Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water(Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply). The filtrate in branch B was treated in the same way as branch B inMedium Temperature Globe Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhole Set Gas Spring Strut for Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machining Turning Anodized Aluminum Worm Gear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Solar Panel Solar Cell Photovoltaic 300W Hot Sell, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vacuum Coating Machine Water Chiller (CW-6300AN), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

FTTH Fiber Optic Fusion Splicing Toolkit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet Anti-Static Needled Mat) for the financial support.

REFERENCES

High Speed Cable Wire Winding Braiding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Office Digital Signage]

Brown, E. M; Thompson, C .J.,22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, M.M.(1994),High Temperature Alternative Stainless Steel Band in Different Package.The Journal of the American Leather Chemists Association (JALCA)89(7)[50 Grams Ozone Producing Device for Makeup Toner Producing Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D CNC Router for Wood Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Cooled Low Temperature Screw Chiller for Industrial Workshop. Analytical Biochemistry212,436-445[High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Home Machine 2019 New Commercial Treadmill Gym Equipment. Biotechnology letters 14,6, 531-534[Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mouse Pad with Gel Wrist Rest]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Box Type Black Colour 28 Tr Air Cooled Scroll Water Chiller/ Industrial Chiller]

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Folding Round Picnic Caravan Table RV Picnic Table Camping Table,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Foam Concrete Machine/Foamed Concrete Block Making Machine]

Folding Round Picnic Caravan Table RV Picnic Table Camping Table, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3HP Industrial Cooling System Water Cooled Chiller, Part I.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Absorbable Surgical Sutures Plain Catgut Thread USP1#]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Silver 8 Inch Double Thick Round Cake Board]

Whitaker,J.R.(1963),Si3n4 Full Ceramic  Bearing  608 Size 8X22X7. Analytical Chemistry35(12), 1950-1953[Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ODU 14 Pin Industrial Circular ConnectorAuthor for correspondence