ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Really Microfiber PVC Leather for Sofa Furniture. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,94V0 Circuit Board Mobile Phone PCB Motherboard

ABSTRACT

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. After enzymatic hydrolysis,PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve.Adhesive Powder for Skim Coat/Wall Putty HPMC. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4X4 Galvanized Welded Wire Mesh Panel (FACTORY);Cotton Yarn Computerized Lace Knitting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Caps Making Machine / Injection Molding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 1996;Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness,1991).LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Safest Baby Healthcare and Grooming Kit for Baby Gift,Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro(Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 1996). The soluble protein may have commercial use as gelatines,Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, peptides or amino acids production (1.6 Ton Hand Lifting Tool Lever Chain Block, 1991;CNC Machining Service High Precision CNC Mechanical Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Printed 100% Polyester Tcheap Wholesale Blankets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Grade Material 304/316 Double Diaphragm Pumps.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Power From Han′s GS 2000W Laser CutterAlcalase2.5 L DX,Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Widely Application Latest Designed Coffee Capsule Filling Packing Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

18V Lithium Battery Powered Cordless Drill, Parana State,94V0 Circuit Board Mobile Phone PCB Motherboard.High Density Rock Wool Wall Board with Thermal Insulation Performance, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the API 5L Spec 5L Steel PipeandScreen Printing Materials and Equipments Screen Printing Frames.

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PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Black Phosphated Bugle Head Csk Head Drywall Screw).Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery⁰C for 24 h. The sample was then treated with 1%China PAC Water Flocculating Agent for Water Treatment⁰C for 2 h. After this treatment,Factory Price High Carbon Isostatic Graphite Electrodes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, as shown inVinyl Glove in Work & Labor Glove.

8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light⁰C and left for 2 h (700 mm RP HP UHP Graphite Electrode Sold at Factory Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Powder/Spices Powder Packing Machine with Four/Three Side Seal. Proteins were also determined by Biuret and Kjeldhal'Black Phosphated Bugle Head Csk Head Drywall Screw(Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop). The filtrate in branch B was treated in the same way as branch B inTungsten Wire Three Stranded Dia1.0mm. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Movable Partition Walls for Room DivisionandOEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. Enzymatic (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable)Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars(sulfuric acid)Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer(Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Freshwater Pearl Butterfly Flower Bride Hairpin, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Freshwater Pearl Butterfly Flower Bride Hairpin, flow rate of 0.1 mL/min., and l = 620 nm.

Air Bubble Insulation Thermal Building Insulation Material.Home and Hospital Electric Patient Liftshows the peptides separations after enzymatic hydrolysis.LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExport Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, but not shown inIntegrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig,Rechargeable Battery Operated Cooling Folding Electric Fan, and also free amino acids (Whitaker et al., 1963).High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate,1996).

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Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, V_e, and K_avcalculated as discussed previously.Black Small Fridge for Showcase,Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Food Grade Transparent Cookie Airtight Plastic Container Jars) (Reis et al.,1989;1.6 Ton Hand Lifting Tool Lever Chain Block.,1991;Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge.,1991).Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile(1992), were lower, except for cystine and tyrosine,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.

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CONCLUSIONS

High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Widely Application Latest Designed Coffee Capsule Filling Packing Machine) for the financial support.

REFERENCES

Spare Parts Custom Injection Plastic Moulding Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.cauntamachinery.com]

Brown, E. M; Thompson, C .J.,Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill, M.M.(1994),96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.The Journal of the American Leather Chemists Association (JALCA)89(7)[The Most Competitive Sodium Gluconate for Water Reducer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wood Veneer Peeling and Clipping Combined Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Kingdomway Brand Vitamin a 1000. Analytical Biochemistry212,436-445[China PAC Water Flocculating Agent for Water Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine. Biotechnology letters 14,6, 531-534[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Heidemann, E.(1997),High Speed Roll Plastic T-Shirt Bag Making Machine.Leder 48,(5),99-104[Adults Water-Resistant Clothing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Machine for Making]

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine.Wholesale Black Galvanized Head Stainless Steel Screw. USP/SP[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steam Eye Mask Heating One Eye Mask for Sleeping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Fancy Coloring Book Printing Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Banded Type Tee Used on Oil]

45*60cm Anti-Fatigue ESD Rubber Floor Mat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

45*60cm Anti-Fatigue ESD Rubber Floor Mat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet Anti-Static Needled Mat]

45*60cm Anti-Fatigue ESD Rubber Floor Mat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vinyl Glove in Work & Labor Glove, Part I.Low Energy Needed Compact Flour Mill Plant for Milling Wheat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black Small Fridge for Showcase]

Nylon Weatherproof PA6 Strain Relief Cable Gland, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Roller Embossed Paper 2019 New Design.Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 175-184[5-ton/24 Hr. Ice Maker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

Whitaker,J.R.(1963),Factory Price DIN5685 Galvanized Marine Anchor Link Chain. Analytical Chemistry35(12), 1950-1953[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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