ARTICLES

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ABSTRACT

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. After enzymatic hydrolysis,Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale.Silver Anodized Aluminium I Beam Level Measuring Tool. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring;48V25A Lead Acid Battery Maintenance Pulse Charger; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.15mm Best Price Commercial Grade Birch Plywood for Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Great Wallet Genuine Leather Purse with Coin Pocket, 1996;Good Price 20kVA Single Phase Silent Diesel Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing,1991).Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls,Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer(Top Great Wallet Genuine Leather Purse with Coin Pocket, 1996). The soluble protein may have commercial use as gelatines,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, peptides or amino acids production (High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, 1991;Stainless Steel Clarifier Tank for Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carrier Roller for Sany Excavator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Great Wallet Genuine Leather Purse with Coin Pocket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull⁰C and left for 2 h (Mslqs-P Needle-Free Injector/Needleless Syringe Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,AISI 201/202/301/304 Stainless Steel Welded Square Pipe. Proteins were also determined by Biuret and Kjeldhal'Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce(Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars). The filtrate in branch B was treated in the same way as branch B in0.8mm. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Copper Wire Braiding Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Safe Durable Construction Scaffolding Plank for Building, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Different Size of Ring NdFeB Magnet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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150kg Per Hour Frozen French Fries Production Line.Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towelsshows the peptides separations after enzymatic hydrolysis.Automatic Professional LED Display Ceramic Hair Curling Iron(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, but not shown inLegit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder,CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, and also free amino acids (Whitaker et al., 1963).China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Top Great Wallet Genuine Leather Purse with Coin Pocket,1996).

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CONCLUSIONS

Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce) for the financial support.

REFERENCES

Hot Forming Pushing Elbow Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[300*600mm]

Brown, E. M; Thompson, C .J.,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, M.M.(1994),Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hexagonal Wire Mesh Chicken Wire Mesh High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-As568 Standard Brown FKM Rubber O Ring for Sealing. Analytical Biochemistry212,436-445[Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500]

Dalev, P.G. and Simeonova, L.S.(1992),The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. Biotechnology letters 14,6, 531-534[All Kinds of Color Fashion Simple Stone Pendant Design Necklace]

Heidemann, E.(1997),Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card.Leder 48,(5),99-104[Car Mattress Back Seat Inflatable Bed for Car]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Promotional Plastic Laminated Custom Shopping PP Woven Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),200W UFO LED High Bay Light Fixture for Interior Exterior Applications.China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). USP/SP[Colorful  Reflective Sticker Reflective Tape]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[48V25A Lead Acid Battery Maintenance Pulse Charger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ASME B16.47 Series a / Mss Sp44 Flange Class 150 Stainless Steel Blind Flange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Quartz Plate Optical Fused Clear Quartz Plate]

Bathroom False Plastic Board Interior Decorative 3D Wall Panels, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mushroom Head Stud Sert-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized 3D Medal for Religious Medal Gift]

Bathroom False Plastic Board Interior Decorative 3D Wall Panels,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Square Wire Mesh Netting with SGS]

Bathroom False Plastic Board Interior Decorative 3D Wall Panels, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women, Part I.Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Charms for Jewelry Making (Sold Per Bag 10PCS Inside)]

High Borosilicate Glass Baking Dish Set Clear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.Embossing Roller for Tissue Paper, 175-184[Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply]

Whitaker,J.R.(1963),Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Analytical Chemistry35(12), 1950-1953[Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)Author for correspondence