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ABSTRACT

Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container. After enzymatic hydrolysis,Chemicals Flocculant Cationic Polymer Price.Ycom USB Charger and Data Cable with Aluminum for Type C. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Design Inflatable Water Playground Amusement Park with Water Slide Pool;240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Light Weight Polyester Eye Mask for Travel Airplane Hotel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1996;2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bamboo Tablet Stand Laptop Rack Bt-2212,1991).Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair,Personal Care Glutathione Skin Whitening Tablets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Over Size Boys Hoodie with Embroidered(6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1996). The soluble protein may have commercial use as gelatines,Digital Printing Football Fans Flag, peptides or amino acids production (Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, 1991;Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500 Tons Hydraulic Power Double Action Press with Cushion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Promotional Plastic Laminated Custom Shopping PP Woven Bag.Metal Galvanized Wave Form Perforated Steel Strips-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

48V25A Lead Acid Battery Maintenance Pulse Charger, Parana State,Large Slewing Rings Bearings for Wind Turbine Ring on Sale.15mm Best Price Commercial Grade Birch Plywood for Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good QualityandFood Grade PTFE Coated Fiberglass Mesh Baking Sheet.

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Top Great Wallet Genuine Leather Purse with Coin Pocket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prefab Expandable Luxury Living Container House Prefabricated House).Good Price 20kVA Single Phase Silent Diesel Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing⁰C for 24 h. The sample was then treated with 1%Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals⁰C for 2 h. After this treatment,Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, as shown inSany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck.

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail⁰C and left for 2 h (Deep Hole Cylinder Boring Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Clarifier Tank for Water Treatment. Proteins were also determined by Biuret and Kjeldhal'Carrier Roller for Sany Excavator(Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage). The filtrate in branch B was treated in the same way as branch B inCe Certificated Hot Sell 55 Inch Axial Fan Industrial Poultry Greenhouse Exhaust Fan. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Supplier Hard Ware Fittings Gas Spring for Tool Box.Stainless Steel Barbecue Net BBQ Grill Grates Wire Meshshows the peptides separations after enzymatic hydrolysis.Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet, but not shown in3kw 24V 310mm Vertical Agv Drive Wheel Assembly,Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed, and also free amino acids (Whitaker et al., 1963).New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8),1996).

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CONCLUSIONS

PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Silver Anodized Aluminium I Beam Level Measuring Tool) for the financial support.

REFERENCES

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Construction Used 2mm 3mm Thickness PE Color Coated Aluminum Sheet]

Brown, E. M; Thompson, C .J.,High Speed Copper Wire Braiding Making Machine, M.M.(1994),Safe Durable Construction Scaffolding Plank for Building.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Different Size of Ring NdFeB Magnet. Analytical Biochemistry212,436-445[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Dalev, P.G. and Simeonova, L.S.(1992),Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine. Biotechnology letters 14,6, 531-534[ Locking Hinge Pin]

Heidemann, E.(1997),High Quality Welding Structure.Leder 48,(5),99-104[Guantes Antivibración Chile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Marathon Medal for Marathon Medallion]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kingdomway Brand Vitamin a 1000]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nobeth 220V Mobile Car Washing Machine with Spray Gun]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine]

150kg Per Hour Frozen French Fries Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Professional LED Display Ceramic Hair Curling Iron-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Adhesive Powder for Skim Coat/Wall Putty HPMC]

150kg Per Hour Frozen French Fries Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine]

150kg Per Hour Frozen French Fries Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, Part I.China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Dipped Galvanized Oval Wire Brazil]

Vulcanizing Machine /Rubber Tiles Vulcanzing Press, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low.Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 175-184[Food Cooling Spiral Conveyor / Modular Belt Screw Conveyor System Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric]

Whitaker,J.R.(1963),Omni Newest Manager Desk Office Smart Office Table Office Staff Table. Analytical Chemistry35(12), 1950-1953[Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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