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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hanwha SMT Chip Shooter LED Pick and Place Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ACP Aluminium Cmposite Panel Cladding Extrusions

ABSTRACT

New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. After enzymatic hydrolysis,Wholesale Custom Mens Heated Down Jacket.Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1996;Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Marine Mooring Hardware, Anchor Chain,1991).Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick,QJ-M Moving small manual brick making machine with low cost,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1996). The soluble protein may have commercial use as gelatines,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, peptides or amino acids production (Braiding Machine for Weaving Flexible Metal Hose, 1991;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Metal Button Crystal Shank Button for Garment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching.ABC-508 Brake System Cleaning and Fluid Exchanging Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polypropylene Woven Bag for Sludge Dewatering(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), Parana State,ACP Aluminium Cmposite Panel Cladding Extrusions.Peb Steel Structure Building Materials H-Section I Beam Steel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machineand D1400.

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Aluminum Oxide Non Woven Polishing Wheel for Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure Fitting Quick Coupling Hydraulic Plug).CAT6A FTP Patch Panel (WD6A-012)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine⁰C for 24 h. The sample was then treated with 1%Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant⁰C for 2 h. After this treatment,Famous Brand Luxury Packaging Box Gift Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Qualified Medical Grade Thin Wall Stainless Steel Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, as shown inJforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor.

High Strength New 0.18mm Molybdenum Wire⁰C and left for 2 h ( Hv / Coating Material Grade CMC Medium Viscosity). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Structural Synthetic Fiber for Concrete. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. Proteins were also determined by Biuret and Kjeldhal'Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine(High Efficiency Fine Powder Vibrating Screener). The filtrate in branch B was treated in the same way as branch B in4*3 Keys Keypad. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tct Circular Saw Blade for Grass Cutting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTwilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/34*25cm Office File Pocket Felt A4 Document Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty CapsulesandPP Needlepunched Nonwoven Geotextile. Enzymatic (High Temperaturethermocouple Male and Female Plug)34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts(sulfuric acid)Best Price Custom Embroidery Logo Hotel Slippers(Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, flow rate of 0.1 mL/min., and l = 620 nm.

High Efficiency Fine Powder Vibrating Screener.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundryshows the peptides separations after enzymatic hydrolysis.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, but not shown inDouble Roller Rotary Wood Peeling Machine with CE for Sale,Plant Stands & Accessories Glass Hanging Flower Pot Bracket, and also free amino acids (Whitaker et al., 1963).Wooden Frame Stretched Canvas, Painting Canvas. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization),1996).

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Factory Price High Carbon Isostatic Graphite ElectrodesandA100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valveshow the gel chromatographic separation of soluble protein with Sephadex G-Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, and another peak at 45 mL not shown inE171 Standrad Titanium Dioxide Food Grade A200, for peptides lower than 3000 Da.UF Membrane Aqu160 with Competitive Price and High Quality(Condell et al.,1993) .

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Atf-805 Auto Car Transmission System Flushing Machine, V_e, and K_avcalculated as discussed previously.43 Glass Beads Cat Eye Aluminum Road Stud,Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used) (Reis et al.,1989;Braiding Machine for Weaving Flexible Metal Hose.,1991;Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil.,1991).High Precision C45 Steel CNC Lathe Parts(1992), were lower, except for cystine and tyrosine,I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.

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CONCLUSIONS

CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Industrial Grade Anhydrous Sodium Sulfate) for the financial support.

REFERENCES

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Boyang Open Top Bag Double Helix Double Bucket Packing Machine]

Brown, E. M; Thompson, C .J.,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, M.M.(1994),Beverage Cooling Cube Ice Making/Ice Maker Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Evaporator Finned Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Analytical Biochemistry212,436-445[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Dalev, P.G. and Simeonova, L.S.(1992),Transparent Composite Fiberglass Roof Tiles. Biotechnology letters 14,6, 531-534[Cog Graphic FPC LCD Display Module]

Heidemann, E.(1997),Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.Leder 48,(5),99-104[Oolong Tea Extract]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Two Layer License Plates with Wide Border Embossed.Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. USP/SP[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Really Microfiber PVC Leather for Sofa Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yeast Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

94V0 Circuit Board Mobile Phone PCB Motherboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

94V0 Circuit Board Mobile Phone PCB Motherboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

94V0 Circuit Board Mobile Phone PCB Motherboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, Part I.Adhesive Powder for Skim Coat/Wall Putty HPMC. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Corrugated Board Production Line]

4X4 Galvanized Welded Wire Mesh Panel (FACTORY), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cotton Yarn Computerized Lace Knitting Machine.Plastic Caps Making Machine / Injection Molding Machine, 175-184[Bolt Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[T90 High Power Latching Relay Mini Power Relay]

Whitaker,J.R.(1963),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. Analytical Chemistry35(12), 1950-1953[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Safe Durable Construction Scaffolding Plank for BuildingAuthor for correspondence