ARTICLES

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ABSTRACT

Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. After enzymatic hydrolysis,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine.Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Manual Foot Pedal Hydraulic Pump;860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CH Series Pid Intelligent Temperature Controller CH402, 1996;China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Precision Infusion Set with Filter and Needle for Single Use,1991).Low Price Sweep Frequency Response Analyzer for Power Transformer Test. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500,Stainless Steel Mechanic Parts for Lock Accessories with MIM Process,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT(CH Series Pid Intelligent Temperature Controller CH402, 1996). The soluble protein may have commercial use as gelatines,Mining Machinery Sand Vibrating Sieve for River Sand, peptides or amino acids production (Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve, 1991;PRO Audio Loudspeaker PA Speaker System Line Array Speaker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Palet Coil Nail Welding Machine/Nail Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality MPO Singlemode Duplex Fiber Optic Adapter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CH Series Pid Intelligent Temperature Controller CH402, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hotel Bedding.Supplier V Wedge Wire Johnson Water Well Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Outlet Automatic Grain Tower Dryer Drying Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170).Mesh Bag Packing 20kg Pure White Garlic(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Supreme 524 Mv Sea Kayak⁰C for 24 h. The sample was then treated with 1%Trunk Organizer Foldable Water Proof⁰C for 2 h. After this treatment,100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Diesel Generator Parts AVR Ki-Davr-95s for Kipor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gld Brand Padlock Brass Popular in 5 Continents  , as shown inHydrolysis Resistant PU Microfiber Leather for Furniture.

No Lead Bronze Flange Manufacturer⁰C and left for 2 h (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Snsc Fd30 Side Shift New Diesel Forklift 3ton. Proteins were also determined by Biuret and Kjeldhal'Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)(Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger). The filtrate in branch B was treated in the same way as branch B inBadminton Court Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Laptop Bag College Men Backpack Leather Back Pack Bags. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Dog Grooming Rake Dematting Tool Shedding Comb(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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White Cotton Satin Hotel Duvet Cover (DPFB8055).Sinicline 2018 Hot Sell Custom Printing Thank You Cardsshows the peptides separations after enzymatic hydrolysis.Small Cleaning Brush ESD Anti Static Brush ESD Brush Black(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetal Material and Sports Theme Custom Metal Medal, but not shown in34*25cm Office File Pocket Felt A4 Document Bag,PAC /Poly Aluminium Chloride for Water Treatment, and also free amino acids (Whitaker et al., 1963).Aluminum Sheet Shearing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CH Series Pid Intelligent Temperature Controller CH402,1996).

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CONCLUSIONS

Factory Supplied High Strength Marine Anchor Chain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole) for the financial support.

REFERENCES

Humidity Refrigeration Digital Controller Jsd-100+, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cable Stripping Machine Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Elegant Europe Dessign Pillow Top Mattress, M.M.(1994),8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).The Journal of the American Leather Chemists Association (JALCA)89(7)[Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Deep Hole Cylinder Boring Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. Analytical Biochemistry212,436-445[Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage]

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Heidemann, E.(1997),High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.Leder 48,(5),99-104[Recycle Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3kw 24V 310mm Vertical Agv Drive Wheel Assembly]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D CNC Router for Wood Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tcn Sex Toy Vending Machines for Sale S800-10]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bright Steel Pipe High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Big Chocolate Storage Holding Tank]

Stainless Steel Screens / Wedge Wire Filter Element, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Amazon Armor TPU Silicone Phone Case for Honor 10 Lite-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light]

Stainless Steel Screens / Wedge Wire Filter Element,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles]

Stainless Steel Screens / Wedge Wire Filter Element, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EPS Foam Sheet Extrusion Machine for Fast Food Box Making, Part I.Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light.Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 175-184[Modern Bathroom Cabinets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Whitaker,J.R.(1963),YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. Analytical Chemistry35(12), 1950-1953[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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