ARTICLES

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ABSTRACT

Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. After enzymatic hydrolysis,Freshwater Pearl Butterfly Flower Bride Hairpin.Air Bubble Insulation Thermal Building Insulation Material. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh;Rechargeable Battery Operated Cooling Folding Electric Fan; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (21.5′′ Wall Mount Display Capacitive Touch Monitor, 1996;Pagoda Gazebo Tent in Guangzhou for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe,1991).Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Small Fridge for Showcase,Microwave Motion Sensor Module Doppler Radar Detector (HW-M10),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile(21.5′′ Wall Mount Display Capacitive Touch Monitor, 1996). The soluble protein may have commercial use as gelatines,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, peptides or amino acids production (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, 1991;Spare Parts Custom Injection Plastic Moulding Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kingdomway Brand Vitamin a 1000. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (21.5′′ Wall Mount Display Capacitive Touch Monitor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.High Speed Roll Plastic T-Shirt Bag Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Wholesale Black Galvanized Head Stainless Steel ScrewAlcalase2.5 L DX,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-45*60cm Anti-Fatigue ESD Rubber Floor Mat); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Vinyl Glove in Work & Labor Glove, Parana State,Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars.Low Energy Needed Compact Flour Mill Plant for Milling Wheat, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (21.5′′ Wall Mount Display Capacitive Touch Monitor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Metal Button Crystal Shank Button for GarmentandHigh-End Business Magnet Leather Ring Binder Notebook.

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Nylon Weatherproof PA6 Strain Relief Cable Gland(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lower Noise Colled Rolled Ball Screw).Steel Roller Embossed Paper 2019 New Design(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil⁰C for 24 h. The sample was then treated with 1%Factory Price DIN5685 Galvanized Marine Anchor Link Chain⁰C for 2 h. After this treatment,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Level Application Waterproof Plastic Box, as shown inGalvanized Eye Grab Bend Hook.

Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine⁰C and left for 2 h (Chinese Galvanized Rectangular Tube for Buildings Materials). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Water Slide for Indoor Play Structure. Proteins were also determined by Biuret and Kjeldhal'Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder(UF Membrane Aqu160 with Competitive Price and High Quality). The filtrate in branch B was treated in the same way as branch B inFactory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1220*2440mm Concrete Formwork black film faced plywood.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Washami Teeth Care for Whitening Cool Mint Toothpaste. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SHXJ1500 CNC Diamond Wire Stone Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PVC Connector Sleeve Soft Plastic Battery Terminal CoversandDry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball. Enzymatic (Yyh Series One Dimension Tumbling Mixer Mixing Machine)ODU 14 Pin Industrial Circular Connector(sulfuric acid)Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board(21.5′′ Wall Mount Display Capacitive Touch Monitor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Classical New Coming Plastic Female Sunglasses, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Classical New Coming Plastic Female Sunglasses, flow rate of 0.1 mL/min., and l = 620 nm.

OEM Double Sided Rigid PCB Circuit Board Manufacturer.Factory Price DIN5685 Galvanized Marine Anchor Link Chainshows the peptides separations after enzymatic hydrolysis.Robot of World Robotics Competition(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inVertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), but not shown inChina Supplier Steel Machine Bellows Cover Telescopic Shield Cover,Professional Rope/Cable/Wire Braiding Machine with Factory Price, and also free amino acids (Whitaker et al., 1963).Aluminum PCB Circuit Board SMD PCBA LED PCB. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (21.5′′ Wall Mount Display Capacitive Touch Monitor,1996).

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Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral HoseandWidely Application Latest Designed Coffee Capsule Filling Packing Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, and another peak at 45 mL not shown inNeedlle Punched Pet/PP 200G/M2 Non Woven Geotextile, for peptides lower than 3000 Da.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover(Condell et al.,1993) .

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Concrete Cure Spray Concrete Curing Agent, V_e, and K_avcalculated as discussed previously.Automatic Chewing Dog Food Snack Machine,White Ceramic Shot Glass for Sublimation Printing - 1.5oz. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf) (Reis et al.,1989;High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation.,1991;Pagoda Gazebo Tent in Guangzhou for Sale.,1991).Promotional Gifts Custom Printed Silicone Wristband/Bracelet(1992), were lower, except for cystine and tyrosine,Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door.

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CONCLUSIONS

8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (45*60cm Anti-Fatigue ESD Rubber Floor Mat) for the financial support.

REFERENCES

Farrowing Crate Pig Floor Cast Iron Slat Floor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Calcium Chloride 74%]

Brown, E. M; Thompson, C .J.,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, M.M.(1994),High Quality Aluminum Heat Sink for Electronics Computer Electric.The Journal of the American Leather Chemists Association (JALCA)89(7)[1jo4 Hydraulic Adaptors for Hose Fittings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. Analytical Biochemistry212,436-445[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Dalev, P.G. and Simeonova, L.S.(1992),Surprise! Range Hood Free! ! ! Juice Cart. Biotechnology letters 14,6, 531-534[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Heidemann, E.(1997),Steam Eye Mask Heating One Eye Mask for Sleeping.Leder 48,(5),99-104[Uses in Food/Beverage Indusry Mint Crystal Menthol]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric Fireplace PCBA with Double Layers Circuit Board]

OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic French Fries Frying Machine with Low Price.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns. USP/SP[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Whole Set Gas Spring Strut for Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Free Sample Multiple PV Cable Mc4 Lead Connector Solar Connectors]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Union Metal Steel Building and Construction Material]

Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Sale Single Cloth Hanger Hook]

Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display]

Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, Part I.High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Band Resaw for Lumber]

T90 High Power Latching Relay Mini Power Relay, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch.Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, 175-184[Cleaning Rubber Ball Clear Rubber Ball Custom Rubber Ball for Seal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Si3n4 Full Ceramic  Bearing  608 Size 8X22X7]

Whitaker,J.R.(1963),Polyglycolic Acid Absorable Suture. Analytical Chemistry35(12), 1950-1953[High Qualified Medical Grade Thin Wall Stainless Steel Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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