ARTICLES

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ABSTRACT

Air Cooled Split Type Ducted Air Conditioner AC Unit. After enzymatic hydrolysis,Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric.High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Gym Fitness Equipment Adjustable Weight Bench;Factory Sell P7.8 Transparent LED Display Screen; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, 1996;Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim,1991).Charms for Jewelry Making (Sold Per Bag 10PCS Inside). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fused Quartz Powder 325mesh 500mesh 1250mesh,Various Types of Plastic Hooks Mould From Manufacturers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle(Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, 1996). The soluble protein may have commercial use as gelatines,10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, peptides or amino acids production (Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift, 1991;Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Soft Baby Suites Dinosaure Pijamas Kigurumi., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Density Celuca Kitchen Cabinets PVC Foam Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc.E171 Standrad Titanium Dioxide Food Grade A200-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Elbow Forming Machine Gorelocker HVAC Duct Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fruit Plum Cleaning Washing Machine with Brushes Spray. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Jh21 Series Pneumatic Power Press CNC Punching Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coal Fried Steam Boiler for Industry with Double Drum, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Slip Resistant Corrugated Protection Entrance Rubber Floor Mat.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Boardshows the peptides separations after enzymatic hydrolysis.Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSynthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), but not shown inEco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup,T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), and also free amino acids (Whitaker et al., 1963).High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105,1996).

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture) for the financial support.

REFERENCES

Low Pressure PU Filter Gasket Sandal Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Okoume Oku Veneer Natural Veneer Face a B C D Veneer Particle Board Marine Plywood]

Brown, E. M; Thompson, C .J.,Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, M.M.(1994),Flour Making Machine Wheat Maize Flour Mill Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens. Analytical Biochemistry212,436-445[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping. Biotechnology letters 14,6, 531-534[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Heidemann, E.(1997),Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh.Leder 48,(5),99-104[LSZH PVC Single Mode Single Core FTTH Cable Price Optical Fiber]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PAC /Poly Aluminium Chloride for Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable Installation and Ce Certification 20g/Hr OEM Air Purifier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Roller Shutter Window Torsion Springs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eco Friendly Detergent]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Computer Plain Socks Knitting Machine with High Quality-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, Part I.New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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