ARTICLES

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^IICNPq/RHAE/biotechnology-FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Power From Han′s GS 2000W Laser Cutter

ABSTRACT

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. After enzymatic hydrolysis,Widely Application Latest Designed Coffee Capsule Filling Packing Machine.Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;18V Lithium Battery Powered Cordless Drill;High Density Rock Wool Wall Board with Thermal Insulation Performance; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, 1996;China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China PAC Water Flocculating Agent for Water Treatment,1991).Factory Price High Carbon Isostatic Graphite Electrodes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets,Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light(Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, 1996). The soluble protein may have commercial use as gelatines,Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, peptides or amino acids production (Powder/Spices Powder Packing Machine with Four/Three Side Seal, 1991;Black Phosphated Bugle Head Csk Head Drywall Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6 Inch Plastic Breathing Cap for The IBC Valve.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, Parana State,High Power From Han′s GS 2000W Laser Cutter.Freshwater Pearl Butterfly Flower Bride Hairpin, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the 316L)andCatering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant.

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Air Bubble Insulation Thermal Building Insulation Material(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner).LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rechargeable Battery Operated Cooling Folding Electric Fan⁰C for 24 h. The sample was then treated with 1%High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve⁰C for 2 h. After this treatment,21.5′′ Wall Mount Display Capacitive Touch Monitor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pagoda Gazebo Tent in Guangzhou for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, as shown inWashami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSpare Parts Custom Injection Plastic Moulding Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kingdomway Brand Vitamin a 1000, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Electrical PV DC Solar Cableshows the peptides separations after enzymatic hydrolysis.Vinyl Glove in Work & Labor Glove(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, but not shown inHigh Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine,Low Energy Needed Compact Flour Mill Plant for Milling Wheat, and also free amino acids (Whitaker et al., 1963).Nylon Weatherproof PA6 Strain Relief Cable Gland. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask,1996).

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Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), V_e, and K_avcalculated as discussed previously.6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck,High Level Application Waterproof Plastic Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Greenhouse Fixing Film Profile Lock Channel and Spring Wire) (Reis et al.,1989;Powder/Spices Powder Packing Machine with Four/Three Side Seal.,1991;China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery.,1991).Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine(1992), were lower, except for cystine and tyrosine,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.

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CONCLUSIONS

Water Slide for Indoor Play Structure. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable) for the financial support.

REFERENCES

Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manufacturer Of Chinese Inclined Bed Cnc Lathe Manufacturers]

Brown, E. M; Thompson, C .J.,1220*2440mm Concrete Formwork black film faced plywood, M.M.(1994),Washami Teeth Care for Whitening Cool Mint Toothpaste.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. Analytical Biochemistry212,436-445[2ton R Type Electric Wire Rope Hoist with Single Speed]

Dalev, P.G. and Simeonova, L.S.(1992),Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. Biotechnology letters 14,6, 531-534[Peb Steel Structure Building Materials H-Section I Beam Steel]

Heidemann, E.(1997),SHXJ1500 CNC Diamond Wire Stone Cutting Machine.Leder 48,(5),99-104[pushpullconnector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. USP/SP[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Accept OEM New Products Team Various Colors Gold Ribbon]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Kraft Paper Bag With Flat Handle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

Yyh Series One Dimension Tumbling Mixer Mixing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ODU 14 Pin Industrial Circular Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Yyh Series One Dimension Tumbling Mixer Mixing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Yyh Series One Dimension Tumbling Mixer Mixing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, Part I.Classical New Coming Plastic Female Sunglasses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

OEM Double Sided Rigid PCB Circuit Board Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Robot of World Robotics Competition.Professional Rope/Cable/Wire Braiding Machine with Factory Price, 175-184[Cotton Tote Bags Manufacturers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[S Series China Generator Helical-Worm Crushers Reduction Box]

Whitaker,J.R.(1963),Aluminum PCB Circuit Board SMD PCBA LED PCB. Analytical Chemistry35(12), 1950-1953[Pre-Seasoned Cast Iron Baking Stone Cookware]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rainbow Metallized Polyester / Holographic Embossing Metallized Pet FilmAuthor for correspondence