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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Braiding Machine for Weaving Flexible Metal Hose. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking

ABSTRACT

Fashion Metal Button Crystal Shank Button for Garment. After enzymatic hydrolysis,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616).10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching;ABC-508 Brake System Cleaning and Fluid Exchanging Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Polypropylene Woven Bag for Sludge Dewatering, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, 1996;High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Industrial Grade Anhydrous Sodium Sulfate,1991).Professional Factory Supply Design Cat Tree Pet Product. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F),Peb Steel Structure Building Materials H-Section I Beam Steel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Oxide Non Woven Polishing Wheel for Machine(China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, 1996). The soluble protein may have commercial use as gelatines,CAT6A FTP Patch Panel (WD6A-012), peptides or amino acids production (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, 1991;Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Famous Brand Luxury Packaging Box Gift Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Qualified Medical Grade Thin Wall Stainless Steel Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Strength New 0.18mm Molybdenum Wire.Structural Synthetic Fiber for Concrete-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Picc Puncture Care Kit, Parana State,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWomen Fashion Winter V Neck Long Sleeve ClothesandBlack Phosphated Bugle Head Csk Head Drywall Screw.

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AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (700 mm RP HP UHP Graphite Electrode Sold at Factory Price).34*25cm Office File Pocket Felt A4 Document Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Needlepunched Nonwoven Geotextile⁰C for 24 h. The sample was then treated with 1%High Temperaturethermocouple Male and Female Plug⁰C for 2 h. After this treatment,34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Price Custom Embroidery Logo Hotel Slippers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, as shown inHousehold Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop.

High Efficiency Fine Powder Vibrating Screener⁰C and left for 2 h (Movable Partition Walls for Room Division). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plant Stands & Accessories Glass Hanging Flower Pot Bracket. Proteins were also determined by Biuret and Kjeldhal'Wooden Frame Stretched Canvas, Painting Canvas(Home and Hospital Electric Patient Lift). The filtrate in branch B was treated in the same way as branch B inFabric Sofa Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUF Membrane Aqu160 with Competitive Price and High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.43 Glass Beads Cat Eye Aluminum Road Stud⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Precision C45 Steel CNC Lathe Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Plastic Caps Making Machine / Injection Molding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tct Circular Saw Blade for Grass Cutting) for the financial support.

REFERENCES

Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ck6136 Best Chinese Lathe Machine Convensional Heavy Duty High Precision Flat Bed Horizontal CNC Lathe]

Brown, E. M; Thompson, C .J.,Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, M.M.(1994),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.The Journal of the American Leather Chemists Association (JALCA)89(7)[China PAC Water Flocculating Agent for Water Treatment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. Analytical Biochemistry212,436-445[ Machine for Making]

Dalev, P.G. and Simeonova, L.S.(1992),Safest Baby Healthcare and Grooming Kit for Baby Gift. Biotechnology letters 14,6, 531-534[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

Heidemann, E.(1997),Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby.Leder 48,(5),99-104[China Energy Chain]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steam Eye Mask Heating One Eye Mask for Sleeping]

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro.Micro DC 24V Worm Gear Brushless Motor for Medical Equipment. USP/SP[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Banded Type Tee Used on Oil]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.6 Ton Hand Lifting Tool Lever Chain Block: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High precision membrane ultra-filtration ceramic filter MF 0050 T 6030E 0812D made in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pet Anti-Static Needled Mat]

CNC Machining Service High Precision CNC Mechanical Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Black Small Fridge for Showcase]

CNC Machining Service High Precision CNC Mechanical Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

CNC Machining Service High Precision CNC Mechanical Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, Part I.Printed 100% Polyester Tcheap Wholesale Blankets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Food Grade Material 304/316 Double Diaphragm Pumps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, 175-184[Hzs25 Small Concrete Batching Plant]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

Whitaker,J.R.(1963),High Power From Han′s GS 2000W Laser Cutter. Analytical Chemistry35(12), 1950-1953[Beverage Cooling Cube Ice Making/Ice Maker Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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