ARTICLES

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ABSTRACT

Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting. After enzymatic hydrolysis,High Precision Piston Ring Good Shape Ability High Quality Surface.Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Square Decorative Stainless Steel Crimped Wire Mesh, 1996;Mold Injection Molding Plastic Products White PC Housing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sw Type BOPP Packaging Adhesive Tape Coating Machine,1991).Stainless Steel Metric Female Flat Seat Hose Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet,Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi Color Spade Made of Steel and Panting(Square Decorative Stainless Steel Crimped Wire Mesh, 1996). The soluble protein may have commercial use as gelatines,Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, peptides or amino acids production (Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine, 1991;New Condition Hot Sale Type Air Cooled Ice Cube Maker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Hinge Pin, Locking Hinge Pin., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4 Way Stretch Running Gym Shorts Unique Women Short Pants.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-VHDCI Connector, 100p, Solder 0.8mm Pitch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Square Decorative Stainless Steel Crimped Wire Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube.Must 4kw 2HP Pump Power Inverter Ags Bts Available-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Farm Relief Plastic Automatic Air Release Valve).Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)⁰C for 24 h. The sample was then treated with 1%Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600⁰C for 2 h. After this treatment,HDPE Geomembrane Lining Price for Pond Liner Film. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, as shown inKh No Fried Automatic Potato Chips Making Machines.

2.0mm Yellow Markers for Fiber Optic Multi Patch Cord⁰C and left for 2 h (Solar Energy Storad Power Generation System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chinese Galvanized Rectangular Tube for Buildings Materials. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Customized Chilled Cast Iron Roll. Proteins were also determined by Biuret and Kjeldhal'IMTEK High Precision and Rigidity Linear Guides for CNC machinery(PP Needlepunched Nonwoven Geotextile). The filtrate in branch B was treated in the same way as branch B inChina Veneer Spindle Peeling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yashi Touch Screen Smart Interactive Whiteboard for Office. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Fireplace PCBA with Double Layers Circuit Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG MonitoringandAnimal Agricultural Damping Horse Stall Rubber Cow Mat. Enzymatic (CNC Automatic Laser Wood Cutting Machine Price)Yc Pure White Nylon Powder for 3D Printing(sulfuric acid)Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats(Square Decorative Stainless Steel Crimped Wire Mesh, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100W 120W 150W Outdoor Solar LED Street Lighting) for the financial support.

REFERENCES

Factory Direct Sale Single Cloth Hanger Hook, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Healthcare Supply Ocitytimes Tobacco Slim Electric Cigarette 500 Puffs E Cigar]

Brown, E. M; Thompson, C .J.,2 Round Sash Pulley for Chain - Brass (PVD), M.M.(1994),Anodized Aluminum Expanded Metal Mesh Competitive Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Slewing Gear for Tower Crane Slewing Winch Reducer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot DIP Galvanized G2150 D Ring Shackle. Analytical Biochemistry212,436-445[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

Dalev, P.G. and Simeonova, L.S.(1992),Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. Biotechnology letters 14,6, 531-534[Mechanical Seal 101 Type for Pump and Auto]

Heidemann, E.(1997),Round Shape Aluminum PCB and PCBA.Leder 48,(5),99-104[Massage Hair Brush]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Garden Save Energy with Solar Flame Wall Light]

Compression Polyester Light Ankle Support Brace for Youth, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),36PCS LED Gold Matrix Stage Zoom Light.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China. USP/SP[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multifunctional Solar Powered Safety Hammer LED Flashlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chicken Cage Mesh Hexagonal Wire Mesh Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tamper Evidence Sticker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag]

110/220V Handle Mild Steel Welding Machine PCB Board 160A, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel]

110/220V Handle Mild Steel Welding Machine PCB Board 160A,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h]

110/220V Handle Mild Steel Welding Machine PCB Board 160A, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Enamel Cast Iron Mini Casserole Cast Iron Cocotte, Part I.Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Forged Automobile Spare Parts with Customized Drawing]

Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug.Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, 175-184[29476 Axial Roller Bearing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Whitaker,J.R.(1963),CNC System Air Duct Line5 Make Machine for HVAC. Analytical Chemistry35(12), 1950-1953[ 100p]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Sheet Metal Fabrication Switchgear Cabinet FactoryAuthor for correspondence