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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Stainless Steel Filter Strainer. After enzymatic hydrolysis,Granite Cutting Diamond Saw Blades Cutting Blade.Steel Forged Automobile Spare Parts with Customized Drawing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production;Slip Resistant Corrugated Protection Entrance Rubber Floor Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1996;High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder,1991).Mechanical Seal 101 Type for Pump and Auto. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case,Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA(T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1996). The soluble protein may have commercial use as gelatines,2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, peptides or amino acids production (Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine, 1991;Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Pressure PU Filter Gasket Sandal Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flour Making Machine Wheat Maize Flour Mill Machinery.Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), Parana State,Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers.Full Computer Plain Socks Knitting Machine with High Quality, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHexagonal Wire Mesh Chicken Wire Mesh High Qualityand1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.

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Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (All Kinds of Color Fashion Simple Stone Pendant Design Necklace).New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette⁰C for 24 h. The sample was then treated with 1%Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card⁰C for 2 h. After this treatment,OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel, as shown inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.

Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products⁰C and left for 2 h (Colorful  Reflective Sticker Reflective Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Made in China Lqa Strap Banding Tool. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New R&D Wire Cable Feeding Winding Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price(Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display). The filtrate in branch B was treated in the same way as branch B inFig Leaf Extract. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Residence Use 220V 50Hz 12kw Portable silent Diesel Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Roller Shutter Window Torsion Springs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

48V25A Lead Acid Battery Maintenance Pulse ChargerandCarbon Graphite Crucible. Enzymatic (Decorative Aluminum Solid Panel for Commercial Building)Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing(sulfuric acid)Di DIN3352 Double Orifice Automatic Air Release Valve Dn65(T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Magic Hair Twist   Curl Sponge Brush, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Magic Hair Twist   Curl Sponge Brush, flow rate of 0.1 mL/min., and l = 620 nm.

Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Quartz Plate Optical Fused Clear Quartz Plateshows the peptides separations after enzymatic hydrolysis.Geyi Disposable Laparoscopic Visible Optical Trocar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized 3D Medal for Religious Medal Gift, but not shown inStainless Steel Square Wire Mesh Netting with SGS,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding, and also free amino acids (Whitaker et al., 1963).Bimetallic Thermometer Industrial Temperature Gauge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm),1996).

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Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, V_e, and K_avcalculated as discussed previously.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings,Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Liquid Spouted Stand up Pouch with Custom Logo) (Reis et al.,1989;Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine.,1991;High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine.,1991).Uprigth Touch-Screen Red Wine Chiller(1992), were lower, except for cystine and tyrosine,Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator.

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CONCLUSIONS

Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Material and Sports Theme Custom Metal Medal) for the financial support.

REFERENCES

Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Silk Twill Scarf]

Brown, E. M; Thompson, C .J.,Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, M.M.(1994),Groove Type Wood Log Tree Peeling and Debarking Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Density Rock Wool Wall Board with Thermal Insulation Performance]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality CE Digital Display Adjustable Power Supply. Analytical Biochemistry212,436-445[White Color Enamel Bowls with High Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires. Biotechnology letters 14,6, 531-534[ALS Stage 5W RGB Animation Laser Light]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Di DIN3352 Double Orifice Automatic Air Release Valve Dn65]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[50 Grams Ozone Producing Device for Makeup Toner Producing Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D CNC Router for Wood Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Office Desk And Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck]

Custom Striped Compression Soccer Socks for Adults, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail]

Custom Striped Compression Soccer Socks for Adults,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India]

Custom Striped Compression Soccer Socks for Adults, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, Part I.High Tensile Strength Galvanized Steel Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book]

Whitaker,J.R.(1963),Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application. Analytical Chemistry35(12), 1950-1953[Mouse Pad with Gel Wrist Rest]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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