ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pet and Animal Food Extrusion Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer

ABSTRACT

Hexagonal Wire Mesh Chicken Wire Mesh High Quality. After enzymatic hydrolysis,V-32 New Special UPS Solar Battery Cabinet Box Case.4-14 Manual Staple Gun with Staples for Hand Tools. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Biomass Drum Wood Chipper with Competitive Price, 1996;High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forged Steel Pressure Sealed Bonnet Globe Valve,1991).Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels,15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure(Biomass Drum Wood Chipper with Competitive Price, 1996). The soluble protein may have commercial use as gelatines,80W All in One Outdoor LED Street Light Solar Lamp, peptides or amino acids production (Hot Sale Safety Vinyl PVC Flooring, 1991;Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Horizental Stainless Steel Food Grade Milk Tank for Milking., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi Parameter Small Digital Domestic Water Meter TDS Meter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Biomass Drum Wood Chipper with Competitive Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sanhe FC-2 Poultry Air Vent.Picosecond Laser Tattoo Removal Skin Care Medical Instrument-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, Parana State,Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Biomass Drum Wood Chipper with Competitive Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theXsg Series High-Speed Rotating Dryer Putty Powder Special DryerandEvaporator Finned Tube.

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Explosion-Proof Housing Sealing Dispensing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price).Tube Mills for Thin Wall Tubing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core⁰C for 24 h. The sample was then treated with 1%Car Badge Custom Emblem Auto Logo Sign for Advertising⁰C for 2 h. After this treatment,2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, as shown inCog Graphic FPC LCD Display Module.

Rear Wheel Exchangeable Aluminum Manual Wheelchair⁰C and left for 2 h (2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Graphic 128X128 Dots Cog LCD. Proteins were also determined by Biuret and Kjeldhal'Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen(K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask). The filtrate in branch B was treated in the same way as branch B inLh38me Small Cement Mortar Pump Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tcn Sex Toy Vending Machines for Sale S800-10.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silver 8 Inch Double Thick Round Cake Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Precision Machining Auto Parts Metal Contact(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Paper Sticky Lint Remover Roller/Pet Hair Roller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Safe Durable Scaffolding Trapdoor Plank for Construction, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%TcandChina LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Enzymatic (Various Sublimation Blank Home Supply Fabric Bath Towel etc)High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(sulfuric acid)Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments(Biomass Drum Wood Chipper with Competitive Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, flow rate of 0.1 mL/min., and l = 620 nm.

Best Quality Bending Metal Mould with All Kinds Metal Materials.Eyes- Caring LED Night Light for Nursing Reading with Soft Lightshows the peptides separations after enzymatic hydrolysis.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, but not shown inYAMAHA Yv100II Nozzle Horder Square Head Short Type,Milling Precision Stainless Steel CNC Machinery Parts, and also free amino acids (Whitaker et al., 1963).U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Biomass Drum Wood Chipper with Competitive Price,1996).

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Corrugated Board Production LineandT90 High Power Latching Relay Mini Power Relayshow the gel chromatographic separation of soluble protein with Sephadex G-Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, and another peak at 45 mL not shown inLuxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, for peptides lower than 3000 Da.Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10(Condell et al.,1993) .

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360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), V_e, and K_avcalculated as discussed previously.Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW),Food Machine Cube Ice Machine for Commercial Use. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board) (Reis et al.,1989;Hot Sale Safety Vinyl PVC Flooring.,1991;High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags.,1991).Christmas Decoration Rose Flower LED Light String(1992), were lower, except for cystine and tyrosine,D Section Stainless Steel Tube.

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CONCLUSIONS

Mint Flavor 50ml Home Foam Toothpaste. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SUS316 Hexagon Socket Set Screws with Cone Point, DIN914) for the financial support.

REFERENCES

125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Euro 3 Outlet Socket Adaptor]

Brown, E. M; Thompson, C .J.,Dxz Limit Switch Tower Crane Spare Parts, M.M.(1994),OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock.The Journal of the American Leather Chemists Association (JALCA)89(7)[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-End Business Magnet Leather Ring Binder Notebook. Analytical Biochemistry212,436-445[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Dalev, P.G. and Simeonova, L.S.(1992),Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Biotechnology letters 14,6, 531-534[ADC12 Aluminum Die Casting Parts]

Heidemann, E.(1997),New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace.Leder 48,(5),99-104[Silencer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Safest Baby Healthcare and Grooming Kit for Baby Gift]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Graphite Sintering Plate Mould for Hard Alloy Sintering]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

The Most Competitive Sodium Gluconate for Water Reducer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

The Most Competitive Sodium Gluconate for Water Reducer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

The Most Competitive Sodium Gluconate for Water Reducer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Magic Hot Hair Styling Curling Iron with Brushes, Part I.Melt Pressure Transducer (PT133). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, 175-184[Celery Juice Powder Benefits]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Whitaker,J.R.(1963),Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. Analytical Chemistry35(12), 1950-1953[100W 120W 150W Outdoor Solar LED Street Lighting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PAC /Poly Aluminium Chloride for Water TreatmentAuthor for correspondence